成纤维生长因子在大鼠后肢骨骼发育早期不同阶段表达的变化及意义

The changes and It＇ s meanings of PGF expressed in lower Limb of the rats in different times in theearly stage of development.

目的通过大鼠肢体发育早期血清学成纤维生长因子（fibroblast growth factors，FGFs）的表达以及后肢标本中碱性成纤维生长因子（base fibroblast growth factor，bFGF）的表达情况，观察其在大鼠后肢肢体骨骼发育早期过程中，组织学上随时问变化的规律。方法将5只清洁级孕鼠的新生鼠分组为A、B、C、D、E五组，分别为10、6、6、8、6、6只。用ELISA法测定各组血清中FGF的浓度，并对其后肢标本进行bFGF的免疫组化染色，从而观察bFGF在关节软骨、骨骺、干骺端及骨膜中的表达情况。结果在新生小鼠的后肢标本免疫组化染色中，bFGF在软骨、骨膜和关节面均有表达，且随出生后时问的变化存在一定的规律：①在关节软骨中bFGF在关节面软骨的表达要强于中心部位的软骨，这种表达随出生后天数增加呈逐渐减弱的趋势。@bFGF在骨骺部增殖层和肥大细胞层以及骨膜内早期表达较强，随时间变化呈逐渐减弱趋势，在出生后10d以后bFGF表达已不明显。③在干骺端成骨细胞内bF-GF的表达不同于它在其他组织内的表达，一直呈现为较高表达，并不随生后时间的延长而呈现减弱趋势。结论在大鼠后肢发育早期，bFGF在后肢肢体各组织中存在良好的表达，提示它在肢体发育过程中与软骨增殖、关节发育与关节内软骨基质的形成和发育有密切的关系。

Objective To explore the FGF expressed in the lower limbs of rats, especially the changes of the expression of bFGF. Through the immunohistochemistry staining combined with orrhology study, to dentify whether the expression of FGF changes with the time goes. Methods The neonatal rats of 5 cleaning SD preg- nant rats were divided into 5 groups. （from group A to E, with 10,6,8,6,6 rats respectively） Through the im- munohistochemistry staining, the expression of FGF- 2 was defined in the tissue （eartilage,periosteum,epiph- ysis） of both sides of the lower limbs of the neonatal rats, the day after birth 1,3,5,10,15 days respectively, and the expressions of FGF in the serum in C,D,E groups（ it was hard to get enough blood in group A and B）. From the experiment , the changes and ranges of the expression of FGF were observed. It can be inferred the value of the changes in the early stage of the development of the limbs. Results According to the experiment, it was identified that there were good expressions of bFGF in lower limbs of the neonatal rats. bFGF is ex- pressed in different kinds of tissues, such as expressed in cartilage, epiphysis, periosteum With the time went on, the expression intensity was reduced. In serology study,it was found that FGF expressed in serum 5 days af- ter the birth,in the same time,there was not found the expression of the FGF in group D and E. And there is significant difference in static analysis between group C ,D and group C ,E（ P 〈 0. 01 ）. And no static difference between group D,E. Conclusions There was good expression in the lower limb tissue during the early stage of the development of the limbs of the rats, which prompts there was great relationship with the development of car- tilage, articulation.