急性胰腺炎不同病理类型基因表达谱差异的研究﹡

Study of gene expression profile disparity in different pathology types of acute pancreatitis

目的:联合应用cDNA微阵列和组织微阵列技术并采用计算机辅助处理技术研究正常胰腺组织、MAP、SAP之间基因表达谱,筛选出MAP与正常粘膜以及MAP与SAP之间的差异表达基因。方法:分别抽提正常胰腺组织、MAP和SAP组织的总RNA并纯化mRNA;将各mR-NA逆转录合成以Cy5和Cy3标记的cDNA一链做探针,分别混合后在3张含有4096条双点人类全长基因的芯片上进行杂交。用ScanArray 4000扫描芯片荧光信号图像,用GenePix Pro 3.0软件对扫描图像进行数字化处理和分析。结果3次杂交出现一致性显著异常,表达差异在2倍以上的基因有141条,其中表达上调的74条,表达下调的67条。结论通过基因表达谱差异的比较,提示MAP和SAP在基因水平存在差异,差异2倍以上的141个基因可能与AP的发生和发展以及相关早期炎症的启动和演化有关。

Objectives： To study the difference of gene expression profile changes in normal pancreas、MAP and SAP tissues by cDNA microarray and to explore the molecular mechanisms in the development of AP.Methods： The cDNA retro-transcribed from equal quantity mRNA from normal pancreas、MAP and SAP tissues which were labeled with Cy5 and Cy3 fluorescence as probes.The mixed probe was hybridized with three pieces BiostarH-40s double dot human whole gene chip.The chips were scanned with a ScanArray 4000.The acquired images were analyzed by using GenePix Pro 3.0 software.Results： A total of 141 genes were screened out that exhibited differentially expression in all three chips.It was identified that in gene expression profiles of MAP there were more than 2 times include 74 upregulated and 67 downregulated genes compared with SAP.Conclusions： A comparison between this two gene profiles shows that： The gene expression level is different in SAP and MAP epithelium.It is shown that these 141 genes probably relate to the occurrence and development of SAP and to the promotion or progress in MAP.