白血病患者RASSF1A基因甲基化状态及其临床意义

RASSF1A gene methylation and its clinical significance in leukemia patients

目的研究白血病患者骨髓中Ras相关区域家族1A(RASSF1A)基因启动子甲基化状态及其临床意义。方法采用甲基化特异性聚合酶链反应(MS-PCR)检测86例白血病患者和60例非白血病患者对照组骨髓中RASSF1A基因启动子的甲基化状况,分析不同白血病类型之间的差异。结果 86例白血病患者中,有12例(13.95%)RASSF1A基因启动子甲基化,而60例非白血病患者骨髓均未检测到甲基化,二者比较差异均有统计学意义(P<0.05)。淋巴系白血病患者RASSF1A基因甲基化比例(23.1%)明显高于髓系白血病患者(6.4%)(P<0.05)。结论白血病患者骨髓中存在RASSF1A基因甲基化,淋巴系白血病甲基化比例明显高于髓系白血病。RASSF1A基因甲基化检测可能成为白血病分型的生物标志物之一。

Objective To research Ras association domain family 1A （RASSF1A） gene promoter region methylation in bone marrow of leukemia patients and its clinical significance. Methods Methylation of RASSF1A gene promoter region was detected by methylation specific polymerase chain reaction （MS-PCR） in the bone marrow of 86 leukemia patients, and the difference of different kinds of leukemia was analyzed. A total of 60 non-leukemia subjects were enrolled as controls. Results Twelve of 86 （ 13.95% ） leukemia patients had RASSF1A gene promoter region methylation. No methylation was found in bone marrow of the control group, and there was a statistical significance （P 〈0.05）. RASSF1A gene methylation ratio in lymphoid leukemia （23. 1% ） was higher than that in myeloid leukemia （6.4%） （P 〈 0.05 ）. Conclusions Leukemia patients have RASSF1A gene methylation. The ratio of RASSF1A gene methyaltion in lymphoid leukemia is obviously higher than that in myeloid leukemia. RASSF1A gene methyaltion may be a significant parameter for diagnosis and genotyping of patients with leukemia.