ICP-AES法测定全血锰的方法学评价

Evaluation on the methodology of blood manganese determination by inductively coupled plasma atomic emission spectrometry

目的对电感耦合等离子体发射光谱法(ICP-AES)测定血锰进行方法学评价,并应用于日常检测工作。方法全血样本经1 mol/L硝酸处理后吸取上清液,采用ICP-AES在279.544 nm处测定样本,评价其精密度、回收率、灵敏度及抗干扰能力;同时采用ICP-AES测定1 027名健康体检儿童的全血锰浓度,确定正常参考范围。结果 ICP-AES测定血锰的批内变异系数(CV)为6.2%～6.6%,批间CV为7.1%～7.6%,总CV为7.2%～7.8%,相对偏差(RSD)为1.66%～2.36%,加标回收率为95.0%～96.9%,线性方程为Y=7 035.6X+759.02,r=0.999 7;在水空白和100μg/L锰标准储备液中按1∶1分别加入25 mg/L钾、钠、氯、钙、镁标准溶液和2 mg/L铁标准溶液后未检测出锰浓度有明显变化。1 027名健康体检儿童的全血锰正常参考范围为(4.89±2.67)μg/L。结论 ICP-AES检测全血锰未发现明显系统误差,且精密度高、准确度好,可作为检测全血锰的常规方法。

Objective To evaluate the methodology of determining blood manganese by inductively coupled plasma atomic emission spectrometry（ICP-AES） , and apply the method in daily detection work. Methods The whole blood samples were treated with nitric acid, and the concentration of the nitric acid was 1 mol/L. The supernatant fluid was collected. The concentrations of the whole blood manganese were determined by ICP-AES under the condition of 279. 544 nm in 1 027 healthy children. The precision, recovery rate, sensitivity and anti-interference ability were evaluated, and the normal reference range was definited. Results The within-run coefficient of variation （CV） was 6.2%-6.6%, the between-run CV was 7. 1%-7. 6%, and the total CV was 7. 2%-7. 8%. The relative standard deviation （RSD） was 1.66%-2.36%. The standardized recovery rate was 95.0%-96.9%. The linear equation was Y = 7 035.6X ＋ 759.02, and the correlation coefficient of the whole blood manganese was r = 0.999 7. The interference elements, K, Na, C1, Ca and Mg （l： 1, 25 mg/L） and Fe （2 mg/L） were added into the blank solution and the standard reserve liquid of 100 Ixg/L manganese. The concentration of manganese had no obvious change. The normal reference range of whole blood manganese in 1 027 healthy children was （4.89 ± 2.67） μg/L. Conclusions ICP-AES for determining whole blood manganese has no obvious system error. This method is accurate and reliable, and can be applied as a conventional way to detect whole blood manganese.