轮状病毒腹泻患儿急性期异形淋巴细胞数量和T淋巴细胞亚群的变化

Changes of the number of atypical lymphocyte and T-lymphocyte subsets in children with acute rotavirus diarrhea

目的研究轮状病毒(RV)腹泻患儿急性期外周血异形淋巴细胞数量和T淋巴细胞亚群的变化。方法采用外周血涂片瑞特染色,显微镜计数40例RV腹泻婴幼儿急性期外周血异形淋巴细胞和20名健康对照组婴幼儿的异形淋巴细胞;同时用流式细胞仪测定T淋巴细胞亚群。结果 RV腹泻患儿急性期外周血异形淋巴细胞计数为(16.18%±3.59%),较健康对照组明显升高(P<0.001);外周血CD3+、CD4+、CD8+T细胞及CD4+/CD8+比值分别为(50.93%±5.86%)、(22.02%±5.17%)、(27.07%±4.79%)及(0.84%±0.29%),较健康对照组明显下降(P<0.01)。结论 RV腹泻患儿急性期外周血异形淋巴细胞数量升高,淋巴细胞亚群异常;RV腹泻患儿急性期进行外周血异形淋巴细胞计数和T淋巴细胞亚群的测定可较好的反映患儿的机体免疫状态。

Objective To investigate dynamic changes of the number of peripheral blood atypical lymphocyte and T-lymphocyte subsets in children with acute rotavirus （RV） diarrhea. Methods A total of 40 children with acute RV diarrhea and 20 healthy controls were enrolled in this study. The proportions of peripheral blood atypical lymphocytes in their peripheral blood smear with Wright stain were figured out by microscopy. Meanwhile, the T-lymphocyte subsets were examined by flow cytometry. Results The proportion of peripheral blood atypical lymphocytes was （ 16.18% -＋ 3.59% ） in children with acute RV diarrhea and had an obviously increase compared with that in healthy controls（ P 〈 0. 001 ）. The percentages of CD3 ~ , CIM ＋ and CD8 ＋ T cells in peripheral blood of children with acute RV diarrhea and CIM ＋/CD8 ＋ ratio were （50.93% -＋ 5.86% ）, （22.02% _＋ 5. 17% ）, （27.07% -＋ 4.79% ） and （0.84% -＋ 0.29% ） respectively. Compared with the healthy controls, the infected groups had a decreased percentage of T cells （P 〈 0.01 ）. Conclusions There are the increasing of atypical lymphocyte number and atypical T-lymphocyte subsets in children with acute RV diarrhea. The determination of atypical lymphocyte number and T-lymphocyte subsets could satisfactorily reflect the immunological state in children with acute RV diarrhea.