摘要
为探讨缺氧诱导因子-2α（HIF-2α）对人结肠癌SW480细胞凋亡的影响，本实验通过培养结肠癌SW480细胞，建立细胞缺氧模型，并通过琼脂糖凝胶电泳和流式细胞术等观察HIF-2a对人结肠癌SW480细胞凋亡的影响。结果显示：（1）单纯缺氧组细胞中HIF-2α表达显著高于常氧组、缺氧＋HIF-2αsiRNA。组和缺氧＋HIF-2asiRNA。组（P〈O．05）；（2）单纯缺氧组与常氧组相比，细胞凋亡明显减少（P〈O．05），常氧组、缺氧＋HIF-2asiRNA。组和缺氧＋HIF-2asiRNA2组之间细胞凋亡数量无明显差异（P〉O．05）。结果表明，HIF-2a可抑制人结肠癌SW480细胞凋亡。
The objective of this study was to explore the influence of hypoxia inducible factor--2α（HIF-2α） on the apoptosis of SW480 cell of human colonic carcinoma. In this experiment culture colonic carcinoma SW480 cells, establishing hypoxia model and observeing the influence of HIF-2α on SW480 cells through agarose gel electrophoresis and flow cytometry were done. As results, 1）in simple hypoxia group the ex-pression of HIF-2α was higher than other three groups（i, e normal oxygen group, hypoxia＋ HIF-2α siR-NA1 group and hypoxia-＋- HIF-2α siRNA2 group） （ P 〈 0.05） ; 2） compared with normal oxygen group, the cell apoptosis of simple hypoxia group was significant less（ P〈0.05）, but among the later three groups cell apoptosis there was no significant change（ P 〉0.05）. Results show that HIF-2α can inhibit the apop-tosis of SW480 cell of human colonic carcinoma.
出处
《中国肛肠病杂志》
2012年第6期9-12,共4页
Chinese Journal of Coloproctology
