IL-10抑制大鼠炎性肉芽肿摄取^(18)F-FDG分析

IL-10 suppresses inflammatory granuloma uptake of ^(18)F-FDG in rats

目的:研究大鼠白介素-10(IL-10)对SD大鼠白细胞和炎性肉芽肿18F-脱氧葡萄糖(18F-FDG)摄取的抑制情况。方法:8只大鼠白细胞分别分成等量两份。其中一份加入0.2 mL IL-10(0.1 mg/mL),另一份不加。水浴中静置120 min后,两份均加18F-FDG 1.85 MBq,60 min后PBS洗涤离心,测量沉淀计数。计算白细胞摄取率。采用弗氏完全佐剂和牛血清白蛋白建立大鼠炎性肉芽肿模型。8只大鼠按10μg/kg经尾静脉注射IL-10,60 min后每只大鼠注入18F-FDG约7.4 MBq,60 min后PET显像。第2天不注射IL-10下进行同样显像。对肉芽肿组织进行病理学检查。结果:在体外试验中,8组SD大鼠血白细胞配对组中,未加入IL-10的白细胞组摄取率为(50.3±6.7)%,加入IL-10的摄取率为(34.6±3.5)%,差异有统计学意义(t=8.9,P<0.01);平均抑制率为31.2%。在IL-10干预下的8只大鼠炎性肉芽肿18F-FDG显像试验中,在注射和未注射IL-10的情况下,其标准摄取值(SUV)分别为1.7±0.4和2.1±0.3(t=20.6,P<0.01)。平均抑制率为19.5%。结论:IL-10可以降低大鼠炎性肉芽肿的糖代谢。

Objective： To evaluate the effectiveness of IL-10 in suppressing lSF-FDG uptake in the inflammatory granuloma of SD rats. Methods： Eight SD rats were killed, and their blood was collected sterily. After centrifugion, the white blood cells were incubated in PRMI 1640 for 3 days. 18F-hen each culture flask of white blood cells was divided into two equal parts. To one group was added 0.2 mL IL-10 solution （0.1 mg/mL）; to the control was added with 0.2 mL of 0.9% sodium chloride solution. All cells were then incubated for 120 minutes at 37℃, after which lSF-FDG （1.85 MBq） was added. Sixty minutes later, the cells were washed twice with PBS and the extent of uptake l8F-FDG determined. In vivo, an inflammatory granuloma was produced by hypodermic injection of rats with a mixture of Freund＇s complete adjuvant, bovine serum albumin and talcum powder. Each rat was maintained for 8 weeks. Imaging of the inflammatory granulomas was performed using the l8F-FDG signal. IL-10 was injected into SD rats at 10 8g/kg of body weight. Sixty minutes later, 7.4 MBq of 18F-FDG were injected, and, after a further 60 minutes, the rats underwent a PET-CT scan. q-he region of interest （ROI） of the inflammatory granuloma was delineatedand the standard uptake value （SUV） calculated. A second PET-CT scan was done without IL- 10 on the next day. q-he granulomatous tissue underwent pathological examination. Results： In the intro test, the with blood cell uptaking ratio of 18F-FDG was （50.3±6.7）% without IL-10, and （34.6±3.5）% with IL-10（t=8.9, P〈0.01）. IL-10 suppressed the rat white blood cell uptaking lSF-FDG. In the PET-CT scan, the SUV of ROI on inflammatory granuloma was 1.7±0.4 with IL-10 and 2.1±0.3 without IL-10 （t=20.6, P〈0.01）. IL-10 suppressed the inflammatory granuloma uptaking lSF-FDG. Conclusion： IL-10 can suppress the inflammatory granuloma of SD rats uptaking lSF-FDG.