摘要
目的研究1,25-二羟维生素D3对糖尿病肾病大鼠肾小管上皮细胞转分化的影响及其可能机制。方法 28只SD大鼠随机分为正常对照组(N组,8只)、糖尿病肾病模型组(M组,10只)、1,25-二羟维生素D3干预组(D组,10只)。检测各组大鼠24-h尿蛋白(24-h Upro)、血尿素氮(BUN)、血清肌酐(SCr)水平,HE染色观察肾组织病理学变化,应用免疫组化、RT-PCR方法分别检测大鼠肾组织α-平滑肌肌动蛋白(α-SMA)和上皮细胞钙黏素(E-cad)的蛋白及其mRNA的表达。结果与N组相比,M组大鼠肾小管间质损伤加重,24-h Upro及SCr水平、α-SMA蛋白及mRNA表达增加,E-cad蛋白和mRNA表达减少(P<0.05);而D组能明显逆转M组上述各观察指标的改变过程(P<0.05)。结论 1,25-二羟维生素D3可能通过下调糖尿病肾病大鼠肾组织α-SMA表达,上调E-cad表达,从而抑制肾小管上皮细胞转分化,减轻肾小管间质病理损伤而发挥其肾脏保护作用。
Objective To investigate the effect and possible mechanism of 1,25-dihydroxy- vitamin Eh on the transdifferentiation of renal tubular epithelial cells in rats with diabetic nephropathy （DN）. Methods Twenty-eight SD rats were randomly divided into three groups of N （normal controls, 8 rats）, M （streptozotocin-induced DN model, 10 rats） and D （DN model rats treated with 1,25-dihydroxyvitamin Eh ）. The levels of 24-hours urinary protein（24 h Upro）, blood urea nitrogen （BUN） and serum creatinine（SCr） were measured, and the pathological changes of kidney tissues were observed by HE staining. The protein and mRNA expressions of a-smooth muscle actin （a-SMA） and E-cadherin （E-cad） in the kidney were detected by immunohistochemistry and RT-PCR, respectively. Results Compared with group N, the injury of renal tubular interstitium was more serious,the levels of 24-h Upro and SCr and the protein and mRNA expressions of a-SMA were increased, whereas the protein and mRNA expressions of E-cad were decreased in group M （P〈0. 05 ）, which were all significantly reversed in group D （P〈0. 05 ）. Conclusion 1, 25- Dihydroxyvitamin D2 has a protective effect on the kidney of rats with DN, which may be related to that 1,25-dihydroxy-vitamin D3 can inhibit the transdifferentiation of renal tubular epithelial cells and mitigate the pathological injury of renal tubular interstitium via down-regulation of a-SMA expression and up-regulation of E-cad expression.
出处
《江苏医药》
CAS
CSCD
北大核心
2012年第15期1743-1746,F0003,共5页
Jiangsu Medical Journal
