大鼠阴茎脚交叉损伤对勃起功能障碍的影响

Erectile dysfunction affected by injury in chiasm of crus penis in rats

目的探讨阴茎脚交叉损伤对勃起功能障碍的影响。方法选用生殖期雄性Wistar大鼠135只,采用抓阄法随机分为阴茎脚交叉切断组(n=25)、阴茎脚交叉切断+单侧坐骨海绵体肌切断组(n=25)、单侧坐骨海绵体肌切断组(n=25)、双侧坐骨海绵体肌切断组(n=25)、假手术组(n=25)及正常组(n=10)。选用育龄期未孕雌性Wistar大鼠,随机与上述各组一一配对饲养,分别观察2个月,了解各组雌鼠受孕情况,并通过雌鼠受孕情况间接推断其配对雄鼠勃起功能障碍。结果阴茎脚交叉切断组雄鼠死亡3只,有效配对雌鼠22只,均受孕,受孕率100%;阴茎脚交叉切断+单侧坐骨海绵体肌切断组雄鼠死亡2只,有效配对雌鼠23只,均未受孕,受孕率0%;单侧坐骨海绵体肌切断组雄鼠死亡4只,有效配对雌鼠22只,21只受孕,受孕率95.5%;双侧坐骨海绵体肌切断组雄鼠死亡3只,有效配对雌鼠24只,均未受孕,受孕率0%;假手术组雄鼠死亡2只,有效配对雌鼠23只,均受孕,受孕率100%;正常组无死亡,配对雌鼠10只,均受孕,受孕率100%。正常组、假手术组、单侧坐骨海绵体肌切断组及阴茎脚交叉切断组之间两两比较差异均无统计学意义(P>0.05),阴茎脚交叉切断组与阴茎脚交叉切断+单侧坐骨海绵体肌切断组及双侧坐骨海绵体肌切断组比较差异有统计学意义(P<0.05),阴茎脚交叉切断+单侧坐骨海绵体肌切断组与双侧坐骨海绵体肌切断组比较差异无统计学意义(P>0.05)。结论单纯切断阴茎脚交叉不会导致勃起功能障碍,阴茎脚交叉切断+一侧坐骨海绵体肌切断会导致勃起功能障碍。

in rats. Methods Objective To investigate the effect of cutting chiasm of crus penis on erectile dysfunction One hundred and thirty-five male Wistar rats at procreative period were randomly divided into a chiasm of crus penis cutting group （ n = 25 ）, a chiasm of crus penis ＋ one-side ischiocavernosus cutting group （n = 25 ）, a one-side ischiocavernosus cutting group （n = 25 ）, a double-side ischiocavernosus cutting group （n =25）, a sham operation group （n =25） and a normal group （n = 10）. Another 135 female Wistar rats at procreative period were picked out to match with the male rats one-to-one randomly, and the records of the pregnancy of these female rats were observed after 2 months. Meanwhile, erectile dysfunction of the male rats was inferred from the records of the pregnancy of their matched female rats. Results Three male rats died in the chiasm of crus penis cutting group. The effective paired female rats were 22 and all of them got pregnancy （ 100% ）. Two male rats died in the chiasm of crus penis ＋ one-side ischiocavernosus cutting group. The effective paired female rats were 23, and none of them got pregnancy （0%）. Four male rats died in the one-side ischiocavernosus cutting group. The effective paired female rats were 22, and 21 of them got pregnancy （95.5%）. Three male rats died in the double-side ischiocavernosus cutting group. The effective paired female rats were 24, and none of them got pregnancy （0%）. Two male rats died in the sham operation group. The effective paired female rats were 23, and all of them got pregnancy （ 100% ）. No male rat died in the normalgroup ant] all of the 10 female rats got pregnancy （ 100% ）. The differences between the normal group, sham operation group, one-side isehioeavernosus cutting group and chiasm of erus penis cutting group were not significant （ P 〉 O. 05 ）. The differences were significant when the chiasm of erus penis culling group compared with the chiasm of crus penis ＋ one-side ischiocavemosus cutting group an（] the （｜ou[）le-side isehiocavernosus cutting group （ P 〈 0. （）5） , but the differenees between the chiasm of crus penis ＋ one-side ischioeavernosus cutting group an（l the double-side isehioeavernosus eutting group were nol significant （ P 〉 0.05 ）. Oonclusion Erectile dysflmction can be achieved l）y cutting the ehiasm of crus penis an（] one-side isehiocavernosus rather than merely cutting the chiasm of crus penis.