骨髓间充质干细胞诱导分化为神经干细胞过程中端粒酶的表达

Telomerase expression during induced differentiation of bone marrow mesenchymal stem cells into neural stem cells

背景:端粒酶在细胞分化及活化过程起重要作用。目的:检测骨髓间充质干细胞诱导分化为神经干细胞过程中端粒酶的表达。方法:采用PCR-ELISA端粒酶检测方法检测SD大鼠骨髓间充质干细胞传代培养前6代细胞中端粒酶的表达;将传代培养的第3代SD大鼠骨髓间充质干细胞诱导分化为神经干细胞,传代培养至第6代,检测各代细胞中端粒酶的表达。以人胚肾细胞系293细胞蛋白提取物作为阳性对照,以灭活细胞蛋白提取物为阴性对照。结果与结论:端粒酶在传代培养的前6代骨髓间充质干细胞中呈阳性表达,为阳性对照组的9.4%～30.9%,第3代表达最高;在诱导分化为神经干细胞传代培养的前5代呈阳性表达,为阳性对照组的5.4%～33.1%,第3代和第4代表达最高。说明端粒酶是细胞分化、增殖的一个重要因素。

BACKGROUND： Telomerase plays an important role in cell differentiation and activation OBJECTIVE： To detect the expression of telomerase during induced differentiation of bone marrow mesenchymal stem cells into neural stem cells. METHODS： The expression of telomerase in the former six generations of SD rat bone marrow mesenchymal stem cell subcultures was detected using the method of PCR-ELISA. Passage 3 SD rat bone marrow mesenchymal stem cells were induced to differentiate into neural stem cells and subpassaged till passage 6, and the expression of telomerase in each generation was detected. The protein extract of 293 cells of human embryo kidney cell line was used as the positive control, and protein extract from inactivated ceils was used as the negative control. RESULTS AND CONCLUSION： Telomerase showed a positive expression in the former six generations of bone mesenchymal stem cell subcultures, which was 9.4%-30.9% of the positive control groups, and the highest telomerase expression appeared in the third generation. Telomerase showed a positive expression in the former five generations of subcultures after induced-differentiation into neural stem cells, which was 5.4%-33.1% of the positive control groups. Telomerase expression peaked in the third and fourth generations of subcultures. These results suggest that telomerase is an important factor of cell differentiation and proliferation.