生理范围内静水压力对体外培养的人膀胱平滑肌细胞的影响

Effects of Hydrostatic Pressure in Physiological Range on Bladder Smooth Muscle Cells in Vitro

为探索生理范围内的静水压对体外培养的人膀胱平滑肌细胞(HBSMCs)的影响,实验采用自行设计的静水压加压装置并按压力梯度分组,用HE染色观察细胞形态,用免疫荧光、流式细胞仪、逆转录聚合酶链式反应(RT-PCR)分别检测细胞骨架F-actin、细胞周期、增殖细胞核抗原(PCNA)与基质金属蛋白酶7(MMP-7)。发现各组的细胞形态、骨架、细胞周期和增殖无明显差异,而各组间MMP-7mRNA的表达呈现出随静水压力的升高而增加随后逐渐下降的趋势。因此,我们得出生理范围内持续静水压可能对体外培养的HBSMCs的形态、骨架、周期、增殖无明显影响,而对MMP-7mRNA的表达具有一定的影响。

To explore the effects of the physiological range of hydrostatic pressure on human bladder smooth muscle cells（HBSMCs）cultured in vitro, we used a hydrostatic compression device designed in our laboratory into the exper- iments, which were grouped by varied hydrostatic pressure gradients. Cellular morphology was observed with HE; staining; cytoskeleton F-actin, cell cycle, both proliferating cell nuclear antigen （PCNA） and matrix metalloprotein- ase 7 （MMP-7） were detected respectively with immunofluorescence, flow cytometry and RT-PCR. We found that the proliferation, cytoskeleton and cycle distribution of HBSMCs were not obviously different among the groups of different hydrostatic pressure; however, the mRNA expression of MMP-7 exhibited a trend of first increasing and then declining as the pressure gradually rises. Thus the physiological range of hydrostatic pressure may not have sig- nificant influence on proliferation, morphology, skeleton, and cell cycle of HBSMCs~ but it may have great effect on the expression of MMP-7.