摘要
为观察碱性成纤维细胞生长因子(bFGF)对骨髓间充质干细胞(BMSCs)分化的影响,采用bFGF诱导山羊BMSCs,通过组织学、免疫组织化学和酶联免疫吸附实验(ELISA)等方法检测BMSCs产生的细胞外基质(ECM)包括Ⅰ型胶原和蛋白多糖的情况,初步评价BMSCs做为颞下颌关节(TMJ)盘组织工程种子细胞的可行性。结果表明:经bFGF诱导,BMSCs向成纤维细胞样细胞分化,能够合成糖胺聚糖(GAG)和I型胶原,因此认为BMSCs作为工程化关节盘种子细胞是可行的。
The present paper is aimed to observe the effects of basic fibroblast growth factor(bFGF) on bone marrow mesenehymal stem cell (BMSCs) differentiation. The bFGF was used to stimulate BMSCs and histology, immuno- histochemistry and enzyme linked immunosorbent assay (ELISA) were used to examine the extracellular matrix pro- duced by induced BMSCs, evaluated the feasibility of BMSCs being the seeding cells of temporomandibular joint (TMJ) disc tissue engineering. The results showed that having been induced with bFGF, the BMSCs could differen- tiate into fibroblast-like cells, which could synthesize GAG and collagen type I matrix. So it is feasible for BMSCs as seeding cells for engineered TMJ disc.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2012年第4期732-736,共5页
Journal of Biomedical Engineering
基金
甘肃省国际合作项目资助(0804WCGA127)
甘肃省科技支撑计划项目资助(1104FKCA144)Δ
关键词
碱性成纤维细胞生长因子
骨髓间充质干细胞
颞下颌关节盘
组织工程
Basic fibroblast growth fator (bFGF)
Bone marrow mesenchymal stem cells (BMSCs)
Temporoman-dibular joint (TMJ) disc
Tissue engineering