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碱性成纤维细胞生长因子对骨髓间充质干细胞向颞下颌关节盘细胞分化的影响 被引量：7

Effects of Basic Fibroblast Growth Factor on Bone Marrow Mesenchymal Stem Cell Differentiation into Temporomandibular Joint Disc Cells
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摘要 为观察碱性成纤维细胞生长因子(bFGF)对骨髓间充质干细胞(BMSCs)分化的影响,采用bFGF诱导山羊BMSCs,通过组织学、免疫组织化学和酶联免疫吸附实验(ELISA)等方法检测BMSCs产生的细胞外基质(ECM)包括Ⅰ型胶原和蛋白多糖的情况,初步评价BMSCs做为颞下颌关节(TMJ)盘组织工程种子细胞的可行性。结果表明:经bFGF诱导,BMSCs向成纤维细胞样细胞分化,能够合成糖胺聚糖(GAG)和I型胶原,因此认为BMSCs作为工程化关节盘种子细胞是可行的。 The present paper is aimed to observe the effects of basic fibroblast growth factor（bFGF） on bone marrow mesenehymal stem cell （BMSCs） differentiation. The bFGF was used to stimulate BMSCs and histology, immuno- histochemistry and enzyme linked immunosorbent assay （ELISA） were used to examine the extracellular matrix pro- duced by induced BMSCs, evaluated the feasibility of BMSCs being the seeding cells of temporomandibular joint （TMJ） disc tissue engineering. The results showed that having been induced with bFGF, the BMSCs could differen- tiate into fibroblast-like cells, which could synthesize GAG and collagen type I matrix. So it is feasible for BMSCs as seeding cells for engineered TMJ disc.
作者 苏雪莲 包广洁 康宏
机构地区 西北民族大学国家民委口腔医学重点实验室 兰州大学口腔医学院
出处 《生物医学工程学杂志》 EI CAS CSCD 北大核心 2012年第4期732-736,共5页 Journal of Biomedical Engineering
基金 甘肃省国际合作项目资助(0804WCGA127) 甘肃省科技支撑计划项目资助(1104FKCA144)Δ
关键词 碱性成纤维细胞生长因子 骨髓间充质干细胞 颞下颌关节盘 组织工程 Basic fibroblast growth fator （bFGF） Bone marrow mesenchymal stem cells （BMSCs） Temporoman-dibular joint （TMJ） disc Tissue engineering
分类号 R782.63 [医药卫生—口腔医学]
  • 相关文献

参考文献9

  • 1苏雪莲,康宏.工程化颞下颌关节盘组织的细胞源问题:现状与展望[J].生物医学工程学杂志,2010,27(2):463-466. 被引量：1
  • 2ALLEN K D, ATHANASIOU K A. Effect of passage and topography on gene expression of temporomandlbular joint disc cells[J].Tissue Eng, 2007, 13(1): 101-110.
  • 3MOREAU J E, CHEN J, BRAMONO D S, et al. Growth factor induced fibroblast differentiation from human bone marrow stromal ceils in vltro[J]. Orthop Res, 2005, 23(1):164-174.
  • 4ZHANG L, TRAN N, CHEN H Q, et al. Time-related changes in expression of collagen types Ⅰ and Ⅲ and of tena- scin-C in rat bone mesenchymal stem cells under co-culture with ligament fibroblasts or uniaxial stretching[J]. Cell Tis- sue Res, 2008, 332(1), 101-109.
  • 5FAN H B, LIU H F, LOK S T, et al. Enhanced differentia- tlon of mesenchymal stem cells co-cultured with ligament fi- broblasts on gelatin/silk fibroin hybrid scaffold[J]. Biomate- rials, 2008, 29(8): 1017-1027.
  • 6MOREAU J E, CHEN J S, HORAN R L, et al. Sequential growth factor application in bone marrow stromal cell liga- ment engineering[J]. Tissue Eng, 2005, 11(11-12): 1887- 1897.
  • 7ZHANG L, KAHN C J, CHEN H Q, et al. Effect of uniaxi- al stretching on rat bone mesenehymal stem cell: orientation and expressions of collagen types Ⅰ and Ⅲ and tenasein-C [J]. Cell Biol Int, 2008, 32(3): 344-352.
  • 8WANG Q W, CHEN Z L, PIAO Y J. Mesenchymal stem cells differentiate into tenocytes by bone morphogenetic pro- tein 12 gene transfer[J]. Biosci Bioeng, 2005,100(4) :418- 422.
  • 9DETAMORE M S, ATHANASIOU K A. Evaluation of three growth factors for TMJ disc tissue engineering[J]. Ann Biomed Eng, 2005, 33(3): 383-390.

二级参考文献25

  • 1MAH J. Histoehemistry of the foetal human temporomandibular joint articular disc[J]. Eur J Orthod, 2004,26 (4) : 359- 365.
  • 2DETAMORE M S, HEGDE J N, WAGLE R R, et al. Cell type and distribution in the porcine temporo-mandibular joint disc[J]. Oral MaxiUofac Surg, 2006,64(2):243-248.
  • 3LEONARDI R, MUZIO L, BERNASCONI G, et al. Expression of vascular endothelial growth factor in human dysfunctional temporomandibular joint discs [J]. Arch Oral Biol, 2003,48(3):185-192.
  • 4ALLEN K D, ATHANASIOU K A. Tissue engineering of the TMJ Disc: A review[J]. Tissue Eng,2006,12(5) :1183- 1194.
  • 5ALLEN K D,ATHANASIOU K A. Growth factor effects on passaged TMJ disk cells in monolayer and pellet cultures[J]. Orthod Craniofacial Res,2006, 9(3):143-152.
  • 6THOMAS M,GRANDE D, HAUG R H. Development of an in vitro temporomandibular joint cartilage analog [J]. Oral Maxillofac Surg, 1991,49(8) :854-857.
  • 7ALLEN K D,ATHANASIOU K A. Effect of passage and topography on gene expression of temporo-mandibular joint disc cells[J]. Tissue Eng,2007,13(1),101-110.
  • 8JOHNAS D E,ATHANASIOU K A. Improving culture conditions for temporomandibular joint disc tissue engineering [J]. Cells Tissues Organs,2007,18S(4) :246-257.
  • 9DETAMORE M S,ATHANASIOU K A. Effects of growth factors on temporomandibular joint disc cells[J]. Archives of Oral Biology, 2004, 49(7) :577-583.
  • 10DETAMORE M S,ATHANASIOU K A. Evaluation of three growth factors for TMJ disc tissue engineering[J]. Annals of Biomedical Eng, 2005,33(3) :383-390.

同被引文献87

引证文献7

二级引证文献39

生物医学工程学杂志
2012年 第4期

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