Y透明剂替代二甲苯在病理制片中的应用

Application of Y clearing agent,a substitute of xlylene,in pathological section preparation

背景:寻找替代二甲苯的透明剂,是避免二甲苯中毒的惟一方法。既往使用松节油、TO透明剂、Hisboelear和SolventCNP30等用做透明剂,终因发挥性不好,溶蜡能力差,刺激性气味强烈,资源有限,价格昂贵,酸价太高或抗氧化能力等指标不理想,未能在制片上完全取代二甲苯。目的:探讨Y透明剂用作病理组织石蜡切片透明剂的可行性。方法:取大鼠肠、睾丸、肺、肾脏不同类型组织及临床肝、网膜、脾、肺、肾、皮肤、子宫刮宫物、胎盘、脾或血栓物多种组织做标本,经过组织固定、脱水、透明、浸蜡、包埋、切片、脱蜡、贴片及烤片等步骤,分别用Y透明剂和二甲苯制作组织切片。结果与结论:用Y透明剂制作的组织切片,透明效果优于二甲苯。使用常规苏木精-伊红染色法及特殊染色法的染色效果与常规制片无明显差异。结果可见无毒新型Y透明剂可以完全替代二甲苯用于病理制片。

BACKGROUND： Finding clearing agents replacing xylene is the only way to avoid xylene poisoning. Turpentine, TO clearing, Hisboelear and Solvent CNP30 are used as clearing agents, but they can not completely replace xylene for their poor volatility and melting wax capacity, strong pungent smell, limited resource, expensive price, high acid value and poor antioxidant capacity. OBJECTIVE： To explore the feasibility of Y clearing as the clearing agent for paraffin section of pathological tissues. METHODS： The tissues of the intestine, testicle, lung and kidney from rats as well as the liver, omentum, spleen, lung, kidney, skin, tissues from uterine curettage, placenta, or thrombus from human being were sampled. These samples were treated with many procedures, including fixation, dehydration, clearing, infiltration, embedding, section, dewaxing and patching. And then, tissue sections were prepared by Y clearing agent and xylene, respectively. RESULTS AND CONLUSION： The tissue sections prepared by Y clearing agent were superior to those prepared by xylene. There was no significant difference in staining effects between hematoxylin-eosin staining and special staining These findings suggest that the new-type non-toxic Y clearing agent can completely replace xylene for pathological section.