直径可控阳极氧化铝表面有序纳米孔阵列对脐静脉内皮细胞黏附行为的影响

Ordered nanopore arrays on the surface of diameter-controlled anodic alumina influence the adhesion behavior of umbilical vein endothelial cells

背景:研究表明多种细胞可响应材料表面微米尺度的表面结构。目的:观察阳极氧化铝膜表面纳米孔阵列孔径变化对脐静脉内皮细胞黏附行为的影响。方法:采用二步阳极氧化法在草酸电解液中制备出孔径可控的(25,50,65,75nm),高度有序排列的阳极氧化铝膜。将人脐静脉内皮细胞接种于不同孔径的阳极氧化铝膜表面,观察脐静脉内皮细胞在阳极氧化铝膜表面的黏附生长情况。结果与结论:脐静脉内皮细胞在不同孔径阳极氧化铝表面上均良好黏附,细胞形态正常。MTT法测试结果表明培养4,24h后,脐静脉内皮细胞在较大孔径(75nm)阳极氧化铝膜上的黏附功能明显高于小孔径(25nm)的阳极氧化铝膜(P<0.05)。表明相对于小孔径(25nm)多孔阳极氧化铝膜,较大孔径(75nm)的多孔阳极氧化铝膜能够促进人脐静脉内皮细胞的黏附,初步显示出较好的生物学活性。

BACKGROUND： Studies have shown that many types of cells can influence the surface structure of micrometer scale on material surfaces. OBJECTIVE： To observe the effect of various pore sizes of ordered nanopore arrays on diameter-controlled anodic alumina on the adhesion behavior of human umbilical vein endothelial cells （hUVECs）. METHODS： The order arranged porous anodic alumina （PAA） with controllable pore sizes （25, 50, 65 or 75 nm） was prepared using a two-step anodizing procedure in oxalic acid electrolytes. The hUVECs were seeded on the PAA surface with different pore sizes to evaluate the cellular adhesion behavior on the PAA surface. RESULTS AND CONCLUSION： The hUVECs adhered well on the surfaces of all the PAA specimens and showed a norrna morphology. M-IT assay showed that the hUVECs adhesion rates were significantly higher on the surface of the PAA with larger diameter of 75 nm compared with the PAA with smaller diameter of 25 nm after 4 and 24 hours of culture （P 〈 0.05）. It is indicated that the PAA with larger diameter of 75 nm can promote the cell adhesion compared with the PAA with smaller diameter of 25 nm based on its better biological activity.