丝素蛋白/透明质酸复合多孔支架材料的表征及细胞相容性

Characterization and biocompatibility of silk fibroin/hyaluronic acid composite porous scaffolds

背景：丝素蛋白和透明质酸具备细胞外基质两种主要成分特征,并且有良好的生物相容性.目的：采用冷冻干燥法制备丝素蛋白/透明质酸复合多孔支架,并观察其表征与细胞生物相容性.方法：将丝素蛋白与透明质酸按10∶1 混合通过冷冻干燥法构建丝素蛋白/透明质酸复合多孔支架并观察其表征.将1×108 L-1的第3～5 代大鼠骨髓间充质干细胞接种在丝素蛋白/透明质酸复合多孔支架上观察其细胞生物相容性.结果与结论：采用冷冻干燥法可以成功制备丝素蛋白/透明质酸复合多孔支架,与纯丝素蛋白支架相比,复合支架具有更好的多孔三维结构.此外,支架制备过程中不含有毒溶剂,支持骨髓间充质干细胞的黏附铺展与增殖.说明实验制备的丝素蛋白/透明质酸复合支架的成孔性好,具有良好的细胞生物相容性.

BACKGROUND： Silk fibroin and hyaluronic acid with good biocompatibility have the features of protein and glycosaminoglycan in the extracellular matrixs. OBJECTIVE： To prepare silk fibroin/hyaluronic acid composite scaffolds using lyophilization, and to evaluate their characterization and cellular biocompatibility. METHODS： The silk fibroin/hyaluronic acid composite scaffold was prepared by lyophilization at a ratio of 10：1 and characterized by scanning electron microscope and Fourier Transform infrared spectroscopy. Passages 3 5 bone marrow mesenchymal stem cells from rats at a density of 1 xl08 cells/L were seeded on the silk fibroin/hyaluronic acid composite scaffold to observe cellular biocompatibility by scanning electron microscope and Laser confocal microscope. RESULTS AND CONCLUSION： The silk fibroin/hyaluronic acid composite scaffold was successfully prepared by lyophilization, showing better porous three-dimensional structure compared with pure silk fibroin scaffold. Good cell adhesion, spreading and proliferation behavior on the silk fibroin/hyaluronic acid composite scaffolds were observed indicating that the cellular biocompatibility of the composite scaffolds is preliminarily confirmed.