可塑型脱矿骨基质/海藻酸钠复合材料的制备及细胞相容性

Preparation and cell compatibility of demineralized bone matrix/sodium alginate composite

背景:制备具有良好骨传导、骨诱导活性、细胞相容性,且可任意塑形的骨修复材料,对于不规则骨缺损以及难愈合性骨折的治疗具有重要意义。目的:构建可塑型脱矿骨基质/海藻酸钠复合组织工程骨材料,筛选出最佳复合比例并评价其细胞相容性。方法:选取健康供体皮质骨,经清洗、粉碎、脱钙处理制备脱矿骨基质,以5/5,6/4,7/3,8/2比例与海藻酸钠复合,通过测定离散直径选取合适的比例构建脱矿骨基质/海藻酸钠复合材料。将所制备脱矿骨基质/海藻酸钠材料浸提液与L-929小鼠成纤维细胞复合培养,2,4,7d后经MTT法检测细胞毒性。结果与结论:随着海藻酸钠含量的增加,复合材料黏性变强,水中离散直径逐步减小,当脱矿骨基质/海藻酸钠质量比为7/3时,复合材料可塑性强,不易离散,在37℃水浴中浸泡2h的离散直径为3.7cm,为复合的最佳比例,经MTT法检测,2,4,7d的细胞增殖率分别为91.56%,95.43%,97.47%,细胞毒性级别均为1级。

BACKGROUND： It is important to prepare a bone substitute material with good osteoacusis, osteoinductive activity and cell compatibility that is easily molded, which is of significance to repair irregular bone defects and refractory bone fractures. OBJECTIVE： To prepare demineralized bone matrix/sodium alginate （DBM/ALG） composite material and to screen out the optimal composite ratio, as well as to evaluate its cell compatibility. METHODS： DBM was prepared from the cortical bone of healthy donors by cleaning, grinding and demineralization. According to the ratio of 5/5, 6/4, 7/3, 8/2, the DBM was compounded with ALG, and the DBM/ALG composite material was prepared through screening the optimal ratio by disperse diameter method. L-929 fibroblasts of mice were cultured in the leaching liquor of DBM/ALG, and the cytotoxicity was tested by MTT method at days 2, 4 and 7. RESULTS AND CONCLUSION： With the increase of ALG content, the viscosity of DBM/ALG was strengthened and dispersed diameter was reduced gradually. When the ratio of DBM/ALG was 7/3, DBM/ALG was not discrete easily, and dispersed diameter was 3.7 cm in 37℃ water. The results of MTT showed that cell proliferation rates were 91.56%, 95.43% and 97.47% at days 2, 4 and 7, respectively; while cytotoxicity was in grade 1.