联合检测对类风湿性关节炎的诊断及临床意义

Combining detection in the diagnose to rheumatoid arthritis and clinical significa

目的探讨类风湿因子（RF）、抗环瓜氨酸多肽抗体（Anti—CCP）、抗核抗体（ANA）、抗双链DNA抗体（抗ds—DNA）、抗ENA抗体联合检测对类风湿性关节炎（RA）患者的诊断及临床意义。方法分别检测20例RA患者（RA组）；20例确诊为非RA自身免疫痛患者（非RA患者组）和20例健康体检者（对照组）的RF、Anti—CCP、ANA、抗ds—DNA、抗ENA抗体。RF采用速率散射比浊法、Anti—CCP采用化学发光微粒予免疫检测法，ANA，ds—DNA抗体检测采用间接免疫荧光法，抗ENA抗体检测采用免疫印迹法。结果1．RA组患者的RF的阳性率为55％、And—CCP的阳性率为80％、ANA阳性率分别为65．0％、抗ds—DNA的阳性率为5．0％、抗ENA抗体阳性率为5．0％，五者联合检测的阳性率为95．0％，明显高于对照组（P〈0．01）。2．非RA组患者的RF的阳性率为20．0％、Anti—CCP的阳性率为5．0％、ANA阳性率分别为60．0％、抗ds—DNA的阳性率为5．0％、抗ENA抗体阳性率为30．0％，五者联合检测的阳性率为85．0％，明显高于对照组（P〈0．01）、结论RA患者中RF、AInj—CCP阳性率较高．五者的联合捡测可提高捡出率．对RA病情的鉴别诊断及发展和疗效的观察均有重要的意义。

Objective Study the clinical signifianee of combine detection of anti - cyclic cimdlin peptide antiboday（ anti - CCP） rheuma-toid iaetor （RF）, CCP, ANA, ds-DNA, ENA antibody in the detection of rheumatoid arthritis （RA） patients. Methods 20 cases of RA pa-tients respectively detection （RA group） ; 20 cases were diagnosed as the RA autoimmune disease, patients （not RA patients gnmp） and 20 pa-tients （control group） the healthy check - up of RF, preview CCP, ANA, fight ds-DNA, fight ENA antibodies. RF the rate than scattering turbidity method, the preview CCP chemiluminescence micro particle immunoassays, ANA, ds - DNA antibody test by indirect immunofluo-reseence, resist ENA antibody test by immune imprinting method. Results l. RA patients RF positive rate was 55 % , preview CCP＇ s positive rate was 80%, ANA positive were 65.0%, resistance to ds - DNA positive rate was 5.0%, fight ENA antibody positive rate was 5.0%, the five joint test positive rate was 95.0%, sigrfificanfly higher than those in the control group （P 〈 .01）O. 2. The RA patients RF positive rate was 20.0%, preview CCP＇ s positive rate was 5.0%, ANA positive were 60.0Vk , resistance to ds - DNA positive rate was 5.0% , fight ENA antibody positive rate was 30.0%, the five joint test positive rate was 85.0%, significantly higher than those in the control group （P 〈 .01 ） O. Conclusion IRA patients RF, preview CCP were higher, five of the joint test can improve the detection rate of RA the differential diagnosis of disease and development and curative effect of observation are of great significance.