桂附地黄丸干预主要组织相容性复合物半相合移植小鼠慢性移植物抗宿主病的效应

Effect of Guifu rehmaniae bolus on preventing chronic graft versus host disease in major histocompatibilty complex halpo-identical bone marrow transplantation mice

背景:目前治疗慢性移植物抗宿主病的方法不理想且不良反应大,发现有效干预和治疗慢性移植物抗宿主病的中医药是移植学者共同关注的焦点之一。目的:观察中药桂附地黄丸对主要组织相容性复合物半相合移植小鼠慢性移植物抗宿主病的干预作用。方法:近交系Balb/cH-2d雄性小鼠作为供鼠,CB6F1雌性小鼠为受鼠,建立主要组织相容性复合物半相合造血干细胞移植小鼠的移植模型,24只受鼠按随机数字表法分为2组,桂附地黄丸组给予桂附地黄丸药液0.2mL/只,每天分2次灌胃,从移植后第1天开始用药;空白组给予等量生理盐水灌胃,用至第100天。结果与结论:桂附地黄丸组小鼠死亡5只,空白组小鼠在移植后第86天前全部死亡(P=0.004)。空白组小鼠移植后第35天、桂附地黄丸组移植后第45天开始出现精神萎靡,临床评分空白组显著高于桂附地黄丸组(P<0.05)。根据病理分级结果,桂附地黄丸组小鼠发生慢性移植物抗宿主病多为0～1级,桂附地黄丸组小鼠肝脏、皮肤、小肠组织与空白组相比,均有不同程度的改善。提示桂附地黄丸可有效减轻主要组织相容性复合物半相合移植小鼠慢性移植物抗宿主病的症状和程度,延长小鼠存活时间,改善组织病理。

BACKGROUND： At present, the method for the treatment of chronic graft versus host disease is not ideal due the adverse reactions, founding the traditional Chinese medicine for effective intervention and treatment of chronic graft versus host disease is one of the common focuses of attention for the transplant scholars. OBJECTIVE： To observe effect of Guifu rehmaniae bolus on preventing chronic graft versus host disease in major histocompatibilty complex halpo-identical bone marrow transplantation mice. METHODS： Transplantation models of major histocompatibilty complex halpo-identical hematopoietic stem cells transplantation mice were established by transplanting the hematopoietic stem cells of male Balb/cH-2d mice to female （Balb/cxC57BL/6） F1 H-2d/b （CB6F1） mice. After transplantation, 24 recipient mice were divided into two groups： Guifu rehmaniae bolus group and blank group, mice in the Guifu rehmaniae bolus group were lavaged from the first day after transplantation with 0.2 mL Guifu rehmaniae bolus liquid twice per day; mice in the blank group were lavaged with the same dose of normal saline and lasted for 100 days.RESULTS AND CONCLUSION： Five mice died in the Guifu rehmaniae bolus group, and all mice died in the blank group at 86 days after transplantation （P=0.004）. The mice in the blank group depressed at 35 days after transplantation and the mice in the Guifu rehmaniae bolus group depressed at 45 days after transplantation, and the clinical score in the blank group was significantly higher than that in the Guifu rehmaniae bolus group （P 〈 0.05）. Pathological observation showed that the mice with chronic graft versus host disease in Guifu rehmaniae bolus group mostly classified from 0 to 1 grade. Compared with blank group, the liver, skin and small intestine tissue of the mice in Guifu rehmaniae bolus group were improved. Guifu rehmaniae bolus can relieve symptoms of chronic graft versus host disease in major histocompatibilty complex halpo-identical bone marrow transplantation mice effectively and prolong their survival time, and improve histologic manifestations.