纤维连接蛋白在肾间质纤维化大鼠肾组织中的表达

Expression of fibronectin in the kidney of rats with renal interstitial fibrosis

背景:纤维连接蛋白表达增加在肾间质纤维化疾病进展过程中发挥着的重要作用。目的:观察纤维连接蛋白在肾间质纤维化大鼠肾组织中的表达。方法:72只大鼠随机等分为对照组、假手术组和模型组。模型组建立单侧输尿管梗阻模型;假手术组仅开腹游离左侧输尿管;对照组不做任何处理。结果与结论:与对照组及假手术组比较,模型组大鼠梗阻时间越长,肾小管间质损害程度越重,纤维化越明显,肾组织中纤维连接蛋白、基质金属蛋白酶9和转化生长因子β1表达即明显增多,且随着梗阻时间延长,肾组织中纤维连接蛋白、基质金属蛋白酶9和转化生长因子β1表达呈持续增加趋势,至造模后第14天达到高峰,与同期对照组、假手术组相比差异有显著性意义(P<0.01),说明在大鼠肾纤维化形成过程中,纤维连接蛋白的蛋白表达在肾损伤早期即显著增加,并随肾间质纤维化程度加重而逐步升高,从而促进肾纤维化的发生。而基质金属蛋白酶9的高表达可能是加重肾间质损害的因素之一。

BACKGROUND： The increasing of the fibronectin expression plays an important role in the process of renal interstitial fibrosis. OBJECTIVE： To investigate the expression of fibronectin in the kidney of the rats with renal interstitial fibrosis. METHODS： Seventy-two male Sprague-Dawley rats were randomly divided into normal control group （n=24）, sham-operation group （n=24） and model group （n=24） by touching ball method. Rats in the model group were used to establish the unilateral ureteral obstruction model, the rats in the sham-operation group received the open surgery and the left ureter was freed, and the rats in the normal control group received no treatment.RESULTS AND CONCLUSION： Histological observations of renal tissues revealed that the degree of renal tubulointerstitial fibrosis of rats in the model group was significantly increased when compared with the normal control group and sham-operated group after unilateral ureteral obstruction, and it was increased gradually accompanying with the increasing of obstruction degree. The expression of fibronectin, matrix metalloproteinase-9 and transforming growth factor β1 in renal tissue of model group was significantly increased, and it was increased gradually accompanying with the increasing of obstruction degree, they all reached the maximum at the 14th day after modeling, there was significant difference in the model group when compared with normal control group and sham-operation group （P 〈 0.05）. It indicated that in the process of the renal interstitial fibrosis in unilateral ureteral obstruction rats, the expression of fibronectin was elevated from the early stage of renal injury and positively associated with fibrosis stage, and then promoted the renal fibrosis. The higher protein expression of matrix metalloproteinase-9 might be one of reasons for increasing the renal interstitial fibrosis.