臭氧对兔骨性关节炎的关节软骨及关节液蛋白多糖含量的影响

EFFECTS OF OZONE ON ARTICULAR CARTILAGE AND PROTEIN PROTEOGLYCAN CONTENTS OF SYNOVIAL FLUID IN OSTEOARTHRITIS OF RABBITS

目的:观察臭氧关节腔注射,对兔骨性关节炎的关节软骨及关节液蛋白多糖含量的影响,进一步探讨臭氧治疗骨性关节炎的作用。方法:将24只成年新西兰兔随机分为4组(每组6只):正常组、模型组,假治疗组,臭氧治疗组。造模成功后,臭氧治疗组关节腔注射40μg/ml臭氧(1 ml)治疗,每周一次,连续两周,假治疗组予以等量空气对照。治疗结束后一周取关节软骨标本进行HE染色、甲苯胺蓝染色、抽取关节液检测蛋白多糖的含量。结果:模型组软骨表面变粗糙,有裂纹糜烂和溃疡形成,软骨层变薄,滑膜明显增厚,关节积液增加,HE染色软骨表面碎裂,软骨细胞增生,结构明显紊乱,甲苯胺蓝染色明显减退,关节液蛋白多糖含量增加,与正常组比较差别有统计学意义(P<0.01);假治疗组软骨可见裂隙,大量血管翳形成,甲苯胺蓝染色轻度减退,部分区域失染,关节液蛋白多糖含量继续增高,与正常组比较差别有统计学意义(P<0.01);臭氧治疗组表层细胞轻度增生,结构可辨认,甲苯胺蓝染色不均,表层失染,关节液蛋白多糖含量减少,与模型组及假治疗组比较差别有统计学意义(P<0.01)。结论:兔骨性关节炎关节腔内注射40μg/ml浓度的臭氧(1 ml)可以明显地降低关节液蛋白多糖含量,对早期骨性关节炎软骨有促进修复作用。

Objective： To observe the effects of ozone on articular cartilage and protein proteoglycan （PG） contents of synovial fluid in osteoarthritic rabbits and to explore the mechanism of ozone on osteoarthritis. Method： 24 New Zealand rabbits were randomly and evenly divided into 4 groups： normal group, model group, sham treatment group and ozone treatment group. After the osteoarthritis models were made successfully, ozone treatment group received intra-articular injection of 03 40 pg/ml （1 ml） once a week. for two weeks. Sham treatment group received 1 ml air. The pathological changes of articular cartilage, and the contents of the PG of joint liquid were compared among four groups. Results： In model group, the cartilage surface became rough, with ulcer, and the cartilage layer became mild pinchout. HE staining showed that the surface of cartilage was broken, cells proliferated, and structure was disordered. Toluidine blue staining was significantly decreased, and PG content of synovial fluid increased compared with normal group （P 〈 0.01）; In sham treatment group, cartilage fracture and a large number of pannus were seen. Toluidine blue staining was slightly decreased, partly loss of staining, and PG content in synovial fluid increased compared with normal group （P 〈 0.01 ）. In ozone treatment group, the surface proliferation was seen, and structure was identifiable. Toluidine blue staining was uneven, surface staining was lost, and PG content of synovial fluid decreased compared with model and sham treatment groups （P 〈 0.01）. Conclusion： Injection of 40 μg/ml ozone （1 ml） can reduce PG content of synovial fluid, and promote the repair of cartilage in the early stage of osteoarthritis.