泊沙康唑对临床来源接合菌的体外抗菌活性研究

In vitro activity of posaconazole against 43 clinical isolates of zygomycetous fungi by E-test

目的研究泊沙康唑对临床来源的接合菌体外抗菌活性。方法对临床来源的43株接合菌采用ITS区序列分析进行准确鉴定,采用E-test纸片法研究泊沙康唑对43株菌的体外药物敏感性,观察其MIC值。结果经ITS序列分析鉴定,43株临床来源接合菌中最为常见的是小孢根霉和不规则毛霉(各12株),其次为米根霉(10株),ramosa(3株)。其他菌种分别为ornata、总状共头霉、微小根毛霉、卷曲毛霉、印度毛霉和冻土毛霉各1株。E-test纸片法测得泊沙康唑对毛霉属、根霉属和Lichtheimia spp.的MIC值范围分别为0.19～>32μg/mL、0.19～3μg/mL、0.002～0.38μg/mL,对总状共头霉和微小根毛霉的MIC值分别为1μg/mL和0.19μg/mL。结论泊沙康唑对大部分接合菌有效,不同种属MIC值范围不同,毛霉属真菌的MIC值差异较大。

Objective To investigate the in vitro acvtivity of posaconazole against 43 clinical isolates of zygomycetous fungi.Methods Forty-three clinical strains were re-identified by ITS region sequencing. The minimal inhibitory concentration of posaconazole against 43 clinical isolates of zygomycetous fungi was assayed by E-test.Results Forty-three strains included Rhizopus microsporus （12 isolates）,Mucor irregularis （12 isolates）,R.oryzae （10 isolates）,Lichtheimia ramosa （3 isolates）,L.ornata （1 isolate）,Syncephalastrum racemosum （1 isolate）,Rhizomucor pusillus （1 isolate）,M.circinelloides （1 isolate）,M.indicus （1 isolate） and M.hiemalis （ 1 isolate）. The MIC ranges of posconazole against Mucor spp.,Rhizopus spp. and Lichtheimia spp. in E-test were 0.19-〉32 μg/mL, 0.19-3 μg/mL and 0.002-0.38 μg/mL, respectively. The MIC values of posaconazole against Syncephalastrum racemosum and Rhizomucor pusillus were 1 μg/mL and 0.19 μg/mL, respectively.Conclusion Most isolates of zygomycetous fungi were susceptible to posaconazole. The MIC values of Mucor spp. were relatively variable.