Neuronatin在人体常见肿瘤中的表达及对肿瘤细胞增殖的影响

The Expression of Neuronatin in Common Tumor and its Effect on Proliferation of Tumor Cell

目的:研究印记基因Neuronatin(NNAT)在人体常见肿瘤中的表达情况及其两种编码产物NNATα和NNATβ对肿瘤细胞增殖的影响。方法:运用组织芯片免疫组化技术对一些人体常见肿瘤及其对应正常组织中NNAT的表达进行系统检测;同时利用腺病毒载体技术,将NNATα和NNATβ分别导入人结肠癌细胞系SW620,并用实时细胞分析仪和平板克隆实验分析细胞增殖能力的变化。结果:①免疫组化结果显示:NNAT在食道鳞癌,结肠腺癌,直肠腺癌,胰腺腺癌,乳腺非特殊性浸润性导管癌,宫颈鳞癌,子宫内膜癌,膀胱移行上皮癌,肺鳞癌,皮肤鳞癌以及前列腺腺癌11种肿瘤组织,肾上腺,皮肤,睾丸,脑,骨骼肌,胰腺6种正常人体组织,以及慢性结肠黏膜炎与慢性肝炎2种炎性组织中呈阳性。②体外细胞增殖实验结果显示,NNATα对人结肠癌肿瘤细胞增殖有一定的抑制作用。结论:NNAT基因在多种人体肿瘤组织中表达,其α片段的表达对于结肠癌细胞系SW620增殖具有轻微抑制作用。

Objective： To investigate the Neuronatin （NNAT） expression in varied common human tumor tissue, and the effect of α and β fragments of NNAT gene on proliferation of tumor cell. Methods： Issue mieroarray technology and immunohistochemistry were used. And adenoviral vector carrying α and β fragments of NNAT gene were employed with clone formation experiment and real time cell analyser. Results： NNAT express in 11 kinds of tumor, which include esophageal squamous carcinoma, colon adenocarcinoma, rectal adenocarcinoma, pancreatic adenocarcinoma, specificity of infiltrating ductal carcinoma, cervical squamous cell carcinoma, endometrial carcinoma, transitional cell carcinoma of bladder, lung squamous carcinoma, skin squamous carcinoma and prostate adenoearcinoma, 6 kinds of normal tissue include adrenal, skin, testis, brain and skeletal muscle, pancreas and 2 kinds of chronic inflammatory tissue, colitis and hepatitis tissue. In vitro, experiment results show that α fragment of NNAT suppress slightly the proliferation of tumor cell. Conclusion： NNAT express in many humor, and NNATα suppress the colon tumor cell line SW620 cell＇s proliferation slightly.