触觉剥夺后豚鼠桶状皮质DCX阳性细胞的实验研究

Experimental Study of DCX-positive Cells after Tactile Deprivation of Barrel Cortex Neurons in Guinea Pigs

目的:观察豚鼠单侧触觉剥夺后豚鼠两侧桶状皮质的DCX阳性细胞数量的差别,探讨触觉剥夺对豚鼠桶状皮质神经发生的影响。方法:12只健康豚鼠随机分为2组,每组6只,制作豚鼠单侧(右侧)触觉(胡须)剥夺模型,之后常规饲养1月和2月灌注取材,用免疫组化和免疫荧光双标法观察同一只豚鼠两侧桶状皮质的DCX阳性细胞情况并比较其数量差异。结果:DCX免疫组织化学染色显示两实验组大脑皮层barrel区DCX阳性细胞数实验侧均明显多于对照侧;NeuN免疫组织化学染色显示两实验组动物大脑皮层barrel区两侧NeuN阳性细胞数差别无统计学意义;DCX和NeuN免疫荧光双标染色显示两实验组大脑皮层barrel区均可见双标细胞存在。结论:触觉剥夺后豚鼠两侧桶状皮质的DCX阳性细胞数具有明显差异性,可能是神经再生的表现。

Objective： To explore the effect of unilateral tactile deprivation of guinea pigs on the expression of DCX in barrel cor- tex by observing the differences in the number of DCX-positive cells in both sides, in order to demonstrate the influence of unilateral tac- tile deprivation of guinea pigs＇ neuroregenesis in barrel cortex. Methods： Twelve healthy guinea pigs were randomly divided into 2 groups （6 guinea pigs each group）. Made guinea pigs with unilateral （right） tactile （beard） deprivation model, then took perfusion samples after conventional breeding for 1 month or 2 months, used immunohistochemistry and immunofluorescence double labeling to detect the DCX-positive cells in barrel cortex on both sides of the same guinea pigs and compared their differences. Results： DCX immunohisto- chemistry results showed there were obviously more DCX-positive cells on experimental side than on the control side in the cerebral cortex barrel region in the two experimental groups. NeuN immunohistochemical staining results showed that there were no statistical differences about the NeuN-positive cells between two sides in animal barrel cortex. DCX and NeuN double-labeled immunofluorescence staining shows that double-labeled cells were seen in the cortex barrel region of two experimental groups. Conclusion： After unilateral tactile deprivation, DCX-positive cells in the barrel cortex in guinea pigs showed significant differences on both sides. It may be the perfor- mance of neuroregenesis.