Stathmin在微血管内皮细胞的表达与胶质瘤恶性程度关系

Stathmin Expression in Microvascular Endothelial Cells Is Associated with Glioma Grade

目的:检测Stathmin在正常脑组织及不同级别胶质瘤微血管内皮细胞中的表达情况。方法:利用结合CD105单克隆抗体的免疫磁珠内皮细胞分选系统特异性分选出68例胶质瘤微血管内皮细胞(其中低级别胶质瘤(WHO分级Ⅰ-Ⅱ)24例,高级别胶质瘤(WHO分级Ⅲ-Ⅳ)44例)和20例正常脑组织微血管内皮细胞。应用免疫组化、RT-PCR和Western blot检测Stathmin在胶质瘤微血管内皮细胞和正常脑组织微血管内皮细胞中的表达。结果:免疫组化证实Stathmin在正常脑组织微血管内皮细胞、低级别胶质瘤微血管内皮细胞和高级别胶质瘤微血管内皮细胞的表达百分率分别是20%,66%和95%(P<0.05)。RT-PCR和Western blot法检测显示,Stathmin在胶质瘤微血管内皮细胞中的表达明显增高。低级别胶质瘤组、高级别胶质瘤组分别与正常组比较,均有显著性差异(P<0.01);且低级别胶质瘤组与高级别胶质瘤组比较,有显著性差异(P<0.01),随着胶质瘤恶性程度的增加,Stathmin表达上调,具有统计学意义。结论:Stathmin在脑胶质瘤微血管内皮细胞中表达随肿瘤恶性程度增高而增加,可能为脑胶质瘤的生物治疗提供一个新靶点。

Objective： To investigate stathminin expression in human gliomas and normal brain specimans derived microvessels endothelial cells（GDMEC）.Methods： Microvascular endothelial cells from human gliomas（GBMEC,n=68） and normal brain（NBMEC,n=20） were purified by incubating with magnetic beads coated with anti-CD105 antibody.There were 24 cases of low grade gliomas（WHOⅠ-Ⅱgrade） and 44 high grade gliomas（WHO Ⅲ-Ⅳgrade）.Expression rates and cellular distribution of stathmin in GDMEC and NBMEC were also investigated by immunohistochemistry.Semiquantitative RT-PCR and Western blot were used for comparing the exp-ression of stathmin in GDMEC and NBMEC.Results： Immunohistochemical analysis demonstrated that the rates of stathmin expression in NBMEC,low grade GDMEC,and high grade GDMEC were 20%,66%,and 95%,respectively（P〈0.05）.The expression of stathmin in GDMEC was higher than that in NBMEC.The difference betweenⅠ-Ⅱgrade,Ⅲ-Ⅳgrade and normal human brain tissues were significant（P〈0.01） respectively.The difference betweenⅠ-Ⅱgrade and Ⅲ-Ⅳ grade was significant（P〈0.01）.Conclusions： With the rise of pathological grades of glioma,the expression of stathmin increased,and it may represent a novel tumor-related target for the therapy of malignant gliomas.