乌灵胶囊对抑郁大鼠脑组织中乙酰化H3及5-HTT、TH表达的影响被引量：8

The Effect of Wuling Capsule on the Expression of Acelyl Histone H3,5-HTT and TH in the Brain Tissue of Depressed Rats

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摘要目的:观察乌灵胶囊对抑郁大鼠脑组织中乙酰化组蛋白H3及5-羟色胺受体(5-HTT)、酪氨酸羟化酶(tyrosine hydroxylase,TH)表达的影响。方法:SD大鼠随机分为对照组、模型组及乌灵胶囊低、高剂量组。取大鼠脑组织,分别采用实时荧光定量PCR法和western blot法检测脑组织中乙酰化组蛋白H3、组蛋白H3,5-HTT、TH蛋白和mRNA的表达。结果:乌灵胶囊明显增强大鼠脑组织中乙酰化组蛋白比例和5-HTT、TH蛋白和mRNA的表达(P<0.05)。结论:乌灵胶囊治疗抑郁症的机制可能与升高脑组织乙酰化组蛋白含量,从而促进5-HTT、TH的表达有关。 Objective： To observe the effect of Wuling capsule on the expression of acelyl histone H3,5-HTT and TH in the brain tissue of depressed rats.Methods： Rats were divide into control,model and two doses groups of Wuling capsule.Contents of acelyl histone H3,histone H3,5-HTT and TH mRNA and protein in he brain tissue of depressed rats were measured by realtime RT-PCR and western blot methods,respectively.Results： Wuling capsule could increase the expression of acelyl histone H3,5-HTT and TH mRNA and protein in the brain tissue of depressed rats.Conclusion： Wuling capsule could cure athymia,this maybe concerned with its effect on acelyl histone H3,5-HTT and TH expression.

作者黎功炳雷宁覃树勇龙军陈德书易伟

机构地区重庆市大足区妇幼保健院成都理工大学材料与化学化工学院化工与制药系

出处《现代生物医学进展》 CAS 2012年第19期3642-3644,3617,共4页 Progress in Modern Biomedicine

关键词抑郁 5-HTT TPH 乌灵胶囊组蛋白H3 Athymia 5-HTT TPH Wuling capsule Histone H3

分类号 Q953 [生物学—动物学]

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