苦荞谷胱甘肽转移酶(FtGST)基因的克隆与序列分析

Cloning and Sequence Analysis of the Glutathione S-transferase(FtGST)Gene from Fagopyrum tatarium

采用RACE技术,从苦荞(Fagopyrum tatarium)中克隆得到一个谷胱甘肽转移酶(Glutathione S-transferase protein,FtGST)基因。序列分析表明,FtGST基因全长DNA序列和cDNA序列编码区分别为746 bp和666 bp,DNA序列含有一个长度为80 bp(342-421 bp)的内含子;开放阅读框(ORF)长666 bp,编码221个氨基酸。生物信息学分析表明,FtGST基因推导的蛋白质含有Tau家族典型的底物结合口袋、谷胱甘肽结合位点(G-site)和疏水性底物结合位点(H-site)氨基酸残基,表明FtGST为Tau家族蛋白。

A Glutathione S-transferase （ FtGST ） gene was isolated from Fagopyrum tataricum by RACE method. Sequence analysis showed that the full-length DNA and cDNA of FtGST was 746 bp and 666 bp, respectively. The DNA of FtGST contains one intron, length of 80 bp （ 342-421 bp ） . The open reading frame （ ORF ） of FtGST encodes 221 amino acid residues. Bioinformatics analysis showed that FtCST protein contains some typical amino acid residues of Tan class to constitute substrate binding pocket, glutathione-binding site （ G-site ） and hydrophobic substrate binding site （ H-site ） . All the characterizations prove FtGST belongs to the Tau proteins family.