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HtrA2转基因鼠的建立与鉴定

Construction and Determination of HtrA2 Transgenic Mice
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摘要 旨在建立神经特异性过表达HtrA2转基因鼠。通过质粒重组技术构建神经特异性表达HtrA2质粒pNSE-HtrA2,利用EcoR I及PvuI切割pNSE-HtrA2获得转基因。显微注射转基因到FVB/N受精卵并移植到假孕鼠的输卵管中发育,获得的转基因鼠利用Western blot鉴定特异性过表达情况。结果显示,成功建立神经特异性过表达HtrA2转基因鼠,神经特异性过表达HtrA2达到5.4倍。本研究建立了神经特异性过表达HtrA2转基因鼠,可用于HtrA2在神经系统的作用研究。 It was to construct transgenic mice that specific over-expression of HtrA2 in neurons. The recombinant plasmid pNSE-HtrA2 that containing human HtrA2 cDNA downstream of NSE promoter was constructed and digested with EcoR I and Pvu I to obtain 4.3 kb transgene. Transgenic mice were obtained by microinjecting of transgene and specific over-expression of HtrA2 was detected by Western blot. Result showed the recombinant plasmid pNSE-HtrA2 was successfully constructed. Transgenic mice with specific-overexpression of HtrA2 up to 5.4 times comparison with controls were successfully obtained. Transgenic mice with specific over-expression of HtrA2 were successfully obtained. Functions of HtrA2 in neurons can be further detected using constructed transgenic mice.
作者 柳明杰 沈雁飞
机构地区 辽宁医学院免疫病原生物学教研室
出处 《生物技术通报》 CAS CSCD 北大核心 2012年第7期93-96,共4页 Biotechnology Bulletin
基金 辽宁省科技厅博士启动项目(20111117) 辽宁省教育厅科研项目(L2010253) 辽宁医学院博士科研启动基金项目
关键词 转基因 过表达 HtrA2 转染 原代培养 Transgene Over-expression HtrA2 Transfection Primary culture
分类号 R346 [医药卫生—基础医学]
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参考文献8

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二级参考文献20

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生物技术通报
2012年 第7期

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