摘要
目的:探讨广东地区MDR-TB菌株rpoB基因突变的分子特征。方法:对95例MDR-TB菌株rpoB基因453-564位密码子片段进行PCR-直接测序。结果:95例MDR-TB菌株rpoB基因突变率91.58%。86例为点突变,1例插入突变,未发现缺失。常见位点为531(63.22%)、526(20.69%)、516(9.20%)。其中:单位点突变69例(80.23%),双位点突变16例(18.60%),三位点突变1例(1.17%)。511位点突变常同时伴有其他位点突变(57.14%)。结论:主要突变位点与国内外报道基本相同,但各位点所占比例具有地域差异;联合突变率较高,占19.54%。512位点插入(AGGAGC)突变可能为新突变类型。
Objective To explore the molecular characteristics of rpoB gene of multidrug-resistant Mycobacterium tuberculosis(MDR-TB) isolates in Guangdong.Methods The codon from 453 to 564 in rpoB gene of 95 MDR-TB isolates was sequenced by PCR.Results The mutation rate of rpoB gene of 95 MDR-TB isolates was 91.58%,86 strains were point mutations,one strain was insertion mutation,there wasn't deletion mutation found.The most frequent mutations were in codon 531(63.22%),526(20.69%),and 516(9.20%).Among the mutation strains,69 were single-site mutation(80.23%),16 were double-site mutations(18.60%),1 was tri-site mutation(1.17%).About 57.14% of 511 site mutations were accompanied by other site mutations.Conclusions The major mutation sites of MDR-TB in Guangdong were similar to the results of domestic and international reports,but the proportions of each mutation form had regional differences.High combinative mutation rate(19.54%) and a new insertion form(512,AGGAGC) were found in this study.
出处
《实用医学杂志》
CAS
北大核心
2012年第14期2333-2335,共3页
The Journal of Practical Medicine
基金
广州市医药卫生科技项目重点项目(编号:2009-Zdi-22)
广州市医药卫生科技项目重大项目(编号:2009-ZDa-03)
国家“十一五”传染病防治重大科技专项(编号:2008ZX10003-007)
关键词
耐多药结核菌
RPOB基因
突变
Multidrug-resitance
Mycobacterium tuberculosis
RpoB gene
Mutation