紫杉醇对血管外膜成纤维细胞MAPK/ERK通路的影响

Effect of paclitaxel on vascular adventitial fibroblasts MAPK/ERK pathway

目的观察紫杉醇对血管外膜成纤维细胞MAPK/Erk通路的影响。方法选雄性SD大鼠,贴壁法培养胸主动脉外膜成纤维细胞并鉴定。以0,9 nmol.L-1紫杉醇分别干预,用Western Blot法,分别检测磷酸化ERK1/2的表达并进行比较。结果磷酸化ERK1/2的表达,在9 nmol.L-1较0 nmol.L-1紫杉醇明显降低;9 nmol.L-1紫杉醇组ERK蛋白的磷酸化水平为0 nmol.L-1紫杉醇组的62%(P<0.05)。结论紫杉醇通过抑制血管外膜成纤维细胞MAPK/ERK通路,能延缓冠脉动脉介入术后再狭窄。

Objective To study influence of paclitaxel on adventitial fibroblasts from MAPK/Erk pathway.Methods Adventitial fibroblast（AF） were prepared from rat aortas using explant technique and cultured for passages,0,9 nmol·L-1 paclitaxel respectively interventions,used Western Blot methods to respectively detect expression of phosphorylation of ERK1/2 then to compare and analysis.Results Phosphorylation of ERK1/2 expression of 9 nmol·L-1 paclitaxel group was significantly less than that of 0 nmol·L-1 paclitaxel group.The levels of 9 nmol·L-1 paclitaxel group on ERK1/2 phosphorylation express is 62% of paclitaxel 0 nmol·L-1 group（P〈0.05）.Conclusion Paclitaxel can delay of restenosis after coronary artery intervention with inhibition vascular adventitial fibroblasts MAPK/ERK path.