黑皮质素受体在破骨细胞形成中的作用

Effect of melanocortin receptors on osteoclastogenesis

目的观察由Raw264.7细胞诱导破骨细胞形成过程中黑皮质素受体(MCR)的作用。方法用α-促黑素(α-MSH)及其类似物SHU9119、PT141、THIQ分别处理体外培养的Raw 264.7细胞,并依此分为对照组、α-MSH组、SHU9119组、PT141组、THIQ组和α-MSH加SHU9119组。培养6天后,经抗酒石酸酸性磷酸酶染色后,观察并计数各组破骨细胞形成数目。RT-PCR法测定Raw 264.7细胞表达的MCR种类。结果在Raw 264.7细胞诱导破骨细胞过程中,α-MSH呈剂量依赖性的增加了破骨细胞形成。SHU9119组、PT141组及α-MSH加SHU9119组均显著增加了破骨细胞形成数目(P<0.05);但THIQ处理组对破骨细胞形成无统计学意义(P>0.05)。Raw 264.7细胞表达5种MCR。结论 MCR激动剂能显著增加破骨细胞形成数目,可能主要通过结合MCR1和/或MCR5促进破骨细胞形成。

Objective To investigate the effect of melanocortin receptor（MCR） in the process of osteoclastogenesis which was induced by Raw 264.7 cells.Methods Raw264.7 cells were treated with α-melanocyte stimulating hormone（α-MSH） and its analogues respectively,which were divided into control group,α-MSH group,SHU9119 group,PT141 group,THIQ group and α-MSH＋SHU9119 group.The number of osteoclasts was observed by Tartrate-resistant acid phosphatase（TRAP） staining after 6 days.RT-PCR was used to determine the type of MCR in Raw264.7 cells.Results α-MSH dose-dependently increased osteoclastogenesis.The number of osteoclasts were significantly increased after treated with SHU9119 group,α-MSH＋SHU9119 group and PT141 group（P〈0.05）,but the THIQ group had no significant effect on osteoclastogenesis（P〉0.05）.RT-PCR showed that all of the MCRs were expressed in Raw264.7 cells.Conclusion MCR agonists could markedly increase the number of oateoclasts,and this process might through M1CR and/or MC5R to complete.