抗骨桥蛋白(OPN)和整合素β3抗体中和OPN、整合素β3对小鼠胚泡种植的影响

The effect s of osteopontin and integrin β3 antibodies on mice blastocyst implantation

目的研究骨桥蛋白抗体和整合素β3抗体对小鼠胚泡着床的影响。方法取20只孕4d小鼠,分别在两侧子宫角内注射骨桥蛋白抗体、整合素β3抗体,对照组注射生理盐水,正常妊娠空白对照组未行任何处理,于孕8d处死小鼠,统计胚泡着床数及妊娠率,取子宫观察内膜的组织学变化。结果结果显示骨桥蛋白抗体组小鼠妊娠率为60%,平均胚胎着床数为1.6±1.19,整合素β3抗体组妊娠率为70%,平均胚胎着床数为1.7±1.28,明显低于生理盐水组及空白对照组(90%,10.0±1.63及100%,10.5±1.58),提示骨桥蛋白抗体和整合素β3抗体明显抑制小鼠胚泡着床。结论骨桥蛋白和整合素β3抗体明显抑制小鼠胚泡的着床并导致子宫内膜的形态学变化,这提示骨桥蛋白和整合素β3与子宫内膜容受性获得有关。骨桥蛋白和整合素β3参与了小鼠胚泡的着床。

Inhibitory effect on mice blastocyst implantation was observed after intra-uterus injection of antibodies against osteopontin and integrin β3. [ Methods ] 20 mice （at Day 4 of pregnancy） were respectively in- jected with antibodies against osteopontin or integrin β3. The control group was injected saline solution. No treat- ment served as the blank control group. Mice were sacrificed at Day 8 of pregnancy, and anatomized. The implanta- tion numbers of mice blastocysts were counted. The endometrium morphology was observed under microscopy. IRe- suits ] The results showed that the pregnancy rate was 60% in osteopontin antiserum group, implantation number of mice blastocyst was 1.6±1.19. The pregnancy rate was 70% in integrin β3-antiserum group, implantation number of mice blastocyst was 1.7±1.28. All of pregnancy rate and implantation number of mice blastocyst were significantly low in the osteopontin-antiserum and integrin β3-antiserum group compared with N.S group （90%, 10.0±1.63） and the blank control group （100%, 10.5±1.58）. These suggest that osteopontin-antibody and integrin β3-antibody block mice blastocyst attachment and implantation. [ Conclusion ] Osteopontin-antibody and integrin β3-antibody could block mice blastocyst attachment and implantation, and induced the morphological changes of uterine endometrial. This indicates that osteopontin and integrin β3 are associated with uterine endometrial receptivity at embryo im- plantation. Osteopontin and interin β3 may participate in mice blastocvst anchor into ute.rine_