从虎杖中同时提取分离白藜芦醇和蒽醌类工艺研究

Study on Extraction and Separation of Resveratrol and Anthraquinone from Polygonum cuspidatum Sieb.et.Zucc

以自然发酵后的虎杖为原料,采用甲醇热回流同时提取虎杖中的白藜芦醇和蒽醌类化合物,选择甲醇浓度、料液比、提取次数和提取时间作为考察因素,以白藜芦醇和蒽醌类化合物的含量为指标,通过正交试验,得到白藜芦醇和蒽醌类化合物同时提取的适宜条件为:体积分数98%甲醇,料液比1∶6,提取3次,每次1 h,白藜芦醇的含量1.912%,提取率达90%,蒽醌类化合物的含量为1.504%,提取率达91.15%;粗提物经V(丙酮)∶V(乙醚)=1∶3的混合溶剂纯化,体积分数50%乙醇溶解分离后,分别得到了白藜芦醇和蒽醌粗品,粗品中白藜芦醇的含量达63.72%,蒽醌含量达50.82%。

Resveratrol and anthraquinone were extracted and separated from Polygonum cuspidatum Sieb.et.Zucc.Methanol hot reflux orthogonal test was applied.Mixed solvent was used in purification and 50% ethanol was used in separation.The optimum conditions of extraction were： 98% methanol,solid-liquid ratio 1∶ 6,extracted 3 times and 1h/ each time.As a result,the content of resveratrol was 1.912% and the content of anthraquinone was 1.504%.The extraction efficiency of resveratrol was 90%,and the extraction efficiency of anthraquinone was 91.15%.Acetone：ether 1∶ 3 was used in purifying,then 50% ethanol was used to separate.Resveratrol and anthraquinone content in coarse product was 63.72% and 50.82%,respectively.This study provided experimental evidence for comprehensive development and utilization of Polygonum cuspidatum Sieb.et.Zucc.