枸杞多糖和1,6-二磷酸果糖协同抗运动疲劳作用及其机制

Synergy anti-fatigue effect of lycium barbarum polysaccharide and 1,6-fructose diphosphates and its mechanism

目的:研究枸杞多糖与1,6-二磷酸果糖的协同抗疲劳作用,为改善和促进运动员体力恢复、提高训练和比赛成绩提供科学依据。方法:将140只小鼠随机分为空白对照组(生理盐水)、枸杞多糖组(20mg.kg-1)及1,6-二磷酸果糖组(300mg.kg-1),并将两者按2×2表分组,以负重游泳小鼠作为研究抗运动疲劳实验的小鼠模型。检测各组小鼠力竭游泳时间、肝糖原、肌糖原、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、血乳酸(LAC)和血尿素氮(BUN),分析两者协同抗疲劳的效果和机制。结果:与空白对照组比较,枸杞多糖和1,6-二磷酸果糖组小鼠负重游泳时间延长,两者剂量分别为20mg.kg-1和300mg.kg-1时最佳(P<0.01),两者之间存在交互作用。与空白对照组比较,枸杞多糖组小鼠LDH、SOD活力提高(P<0.01),LAC和血BUN水平降低,1,6-二磷酸果糖使小鼠肝糖原和肌糖原的储备量显著提高(P<0.01)。结论:枸杞多糖与1,6-二磷酸果糖配伍能起到协同抗疲劳作用,其原因可能是1,6-二磷酸果糖增加了小鼠的肝糖原和肌糖原的贮备,能够提供更多能量;枸杞多糖提高LDH、SOD活力,加快代谢产物的分解,使小鼠在耐力运动中坚持时间更长。

Objective To investigate the anti-fatigue effect of lycium barbarum polysaccharide（LBP） and 1,6-fructose diphosphates（FDP） and to provide scientific basis for improving training effectiveness and race performance.Methods 140 mice were randomly divided into saline-negative control group,LBP group（20 mg·kg-1）,FDP group（300 mg·kg-1） and the 2×2 analysis variance groups.The burden swimming mice were used as animal models.The swimming time,liver glycogen,muscle glycogen,lactate dehydrogenase（LDH）,superoxide dismutase（SOD）,blood lactate（LAC） and blood urea nitrogen（BUN） were detected.The synergy effect of LBP and FDp was analyzed.Results Compared with negative control group,the loading swimming time in FDP and LBP groups was prolonged（P〈0.01）,which indicated that they played synergy effect on swimming time.The best doses of LBP and FDP were 20 mg·kg-1 and 300 mg·kg-1（P〈0.01）.The activities of LDH and SOD were increased（P〈0.01） and the levels of LAC and BUN were decreased in LBP group,and the liver glycogen and muscle glycogen of mice were increased in FDP group（P〈0.01）.Conclusion FDP and LBP exhibits a synergy effect on anti-fatigue,its mechanism may be related to that FDP can increase the liver glycogen and muscle glycogen reserve of mice which can provide more energy,and LBP can accelerate the decomposition of metabolites,and make mice persist more time in endurance exercise.