淡色库蚊肌动蛋白全长基因的克隆及生物信息学分析

Cloning and Bioinformatic Analysis of Full-length actin Gene of Culex pipiens pallens

为阐明肌动蛋白抗药性相关机制及研制新型卫生杀虫剂奠定基础,根据库蚊抗性与敏感品系差异表达的EST片段,设计特异扩增引物,运用RACE技术从淡色库蚊抗性品系中扩增出该抗性相关基因的全长cDNA序列,分析其生物信息学特性。结果表明,获得淡色库蚊肌动蛋白基因cDNA全长1 708bp序列,其编码377个氨基酸;该基因编码的蛋白为膜蛋白,具有27个跨膜螺旋、1个信号肽切割位点、27个磷酸化位点。

Culex pipiens pallens is the main carrier of multiple viruses and parasites, and there is close relationship between actin protein and pesticide resistance. Based on gene fragments obtained by resistance-related design reverse transcription and amplification primers, using rapid amplification of cDNA ends method （RACE）, the full length of the gene was amplified from a resistant strain of Culex to analyze their bioinformatic characteristics. The actin gene obtained in Culex pipiens has 1 708 bp coding 377 amino acids. The bioinformatic analysis showed that actin gene was a membrane protein with one helix, one signal peptide cleavage point and twenty-seven phosphorylation sites. The full-length actin gene and biological information lay the foundation for clarifying the resistance mechanism of the actin gene and development of new pesticides.