马勃多糖提取及体外抗肿瘤研究

Polysaccharide Extraction from Calvatia Gigantea and its in Vitro Anti-tumor Activity

目的研究马勃子实体多糖的最佳提取条件及其体外抗肿瘤作用。方法通过单因素及正交试验确定热水浸提法提取马勃多糖(CGP)的最佳提取工艺条件。利用MTT法研究马勃多糖对宫颈癌细胞(Siha)和乳腺癌细胞(MDA)体外增殖的抑制作用。结果通过正交试验确定CGP的最佳提取条件为提取时间:1.5 h、提取次数:2次、提取温度:100℃、料液比:1∶15;体外肿瘤细胞增殖抑制实验结果显示:马勃醇沉多糖在250μg.mL1时对Siha细胞具有最高抑制率达到52.6%;醇溶多糖在250μg.mL1时对MDA的抑制率达到80.4%。结论在最佳提取条件下马勃多糖热水浸提的得率为:1.792%;马勃多糖对Siha及MDA肿瘤细胞具较好的抑制作用。

OBJECTIVE To study the optimal conditions for polysaccharide（CGP） extraction from fruiting bodies of Calvatia gigantea. METHODS First, the single factor and orthogonal tests were used to find the optimum conditions for polysaccharide extraction by hot water. Then, the proliferation inhibition effects on Cervical cancer cells （Siha） and breast cancer cells（MDA） in vitro were tested by MTT method. RESULTS Based on the single factor and orthogonal tests, the optimum extraction conditions were： extraction time 1.5 h, 2 times, temperature 100 ~C and solid-liquid ratio l-15. The in vitro experiments showed that the ratio of proliferation inhibition on Siha cells reached 52.6% when the concentration of alcohol precipitation parts was 250μg·mL-1. Moreover, alcohol-soluble components had inhibition ratio of 80.4% for MDA cells. CONCLUSION Under above conditions, the productivity of polysaccharide extracted from fruiting bodies of Calvatia Calvatia ntean is 1.792%. And CGP has good inhibition effect on proliferations of Siha and MDA cells.