摘要
目的建立输液袋用聚碳酸酯组合盖中双酚A含量及双酚A向输液中迁移量的高效液相色谱测定法。方法采用Diamosil-C18(4.6mm×250mm,5μm)色谱柱;流动相为甲醇-水(80:20),检测波长为227nm,流速1.0mL·min^-1。结果双酚A在1.354-27.08ng内呈良好的线性关系(r=0.9999);聚碳酸酯组合盖中双酚A含量和双酚A向输液中迁移量测定的平均回收率分别为91.7%(RSD=2.3%)和93.9%(RSD=1.8%)。结论该方法准确、灵敏、简便,适用于对输液袋用聚碳酸酯组合盖中双酚A含量及双酚A向输液中迁移量的测定。
OBJECTIVE To develop a method for determination of bisphenol A in Infusion Used Combined Cover and bisphenol A migration into infusion by HPLC. METHODS A Diamosil-C18 column(4.6 mm×250 mm, 5μm) and a DAD detector was adopted with methanol and water(80 : 20) as mobile phase. The wavelength was 227 nm, the flow rate was 1.0 mL.min-1. RESULTS The method had a linear range in 1.354-27.08 ng (r=0.999 9). The average recovery of bisphenol A and bisphenol A migration was 91.7%(RSD=2.3%) and 93.9%(RSD=l.8%), respectively. CONCLUSION This method is accurate, sensitive, simple, and can be used for determination of bisphenol A and bisphenol A migration into infusion from Infusion Used Combined Cover.
出处
《中国现代应用药学》
CAS
CSCD
2012年第7期641-643,共3页
Chinese Journal of Modern Applied Pharmacy