模拟粪便标本中的单增李斯特菌的分离和检测

Isolation and identification of Listeria monocytogenes in artificial fecal samples

目的建立针对粪便标本中单增李斯特菌的分离鉴定方法,评价方法的检测下限,以提高感染人群标本中单增李斯特菌的检出率,了解我国人群中该菌的携带及感染情况。方法对模拟人粪便标本进行二次增菌,采用Real-time PCR检测,并同时分离培养获得该病原菌。结果当每克模拟粪便标本中含有7 cfu的单增李斯特菌时,经过增菌后的标本用Real-time PCR方法可检测出阳性结果,并能够通过单增李斯特菌的选择培养基分离得到病原菌。结论本研究为从粪便标本中分离单增李斯特菌和由单增李斯特菌引起的食物中毒事件的病原学调查提供了技术支持,有利于对我国人群中单增李斯特菌的携带或感染状况进行调查分析。

Objective To establish the isolation and identification assay of Listeria monocytogenes in fecal sample, increase the detection rate and investigate the infection rate of Listeria monocytogenes in population in China. Methods Real-time PCR and traditional detections of Listeria monocytogenes were conducted after tow enrichments of artificial fecal samples, Results When Listeria monocytogenes concentration was 7 CFU/g in artificial fecal sample, the realtime PCR and culture results were positive. Conclusion The result of this study provides laboratory evidence for the isolation and identification of Listeria monocytogenes from fecal samples and etiological survey of food poisoning caused by Listeria monocytogenes, which would facilitate the survey of carriage or infection status of Listeria monocytogenes in population in China.