乳腺癌MCF-7细胞系中侧群细胞分选及其生物学特性

Sorting of side population cells from breast cancer MCF-7 cell line and its biological characteristics

目的分离乳腺癌MCF-7细胞系中的侧群细胞（sP）并观察其生物学特性。方法利用流式细胞荧光分选法将乳腺癌MCF-7细胞系分成SP和非SP细胞两个亚群。对两个亚群细胞采用软琼脂克隆形成实验观察其增殖能力。结果MCF-7细胞株中分选出SP细胞占（6．5±0．4）％；SP细胞的体外克隆形成率为（38．5±9．4）％，高于非sP细胞的（8．4±2．6）％（t=5．34，P〈0．05）。结论乳腺癌MCF-7细胞中的SP细胞富集了乳腺癌干细胞，其增殖能力强于非SP细胞，表明SP表型的肿瘤细胞在乳腺癌的生长中具有重要的地位。

Objective To separate the side population cells （SP） from breast cancer MCF-7 cell line, and observe its biological characteristics. Methods Flow cytometry and Hoechst 33342 dye efflux assay were used to iso- late SP ceils and non-SP ceils from the MCF-7 cell line of human breast cancer. Tumorigenicity of the two snbpopula- tions was observed by a soft agar cloning method. Results The results of FACS analysis indicated that （6.5± 0.4 ） % of the MCF-7 cells were SP cells ; The vitro colony formation rate of SP cells was （ 38.5 ± 9.4） %, and higher than that of non-SP cells （ 8.4± 2.6） % （ t = 5.34, P 〈 0.05 ）. Conclusion The SP ceils sorted from MCF-7 cell line enriched tumor stem cells, which exhibited high tumorigenicity. It indicated that SP cells should play a principal role in breast cancer.