幼儿末梢血放置时间对血常规检测的影响

目的:了解幼儿末梢血放置时间对血常规检测的影响,用以日常工作中合理安排工作流程,为临床提供可靠的实验数据。方法:收集50名2～5岁健康幼儿末梢血以乙二胺四乙酸三钾(EDTA-K3)抗凝,室温中(20～25℃)分别于即刻、0.5小时、2小时、4小时、8小时用全自动血细胞分析仪进行检测。结果:室温下,随着末梢血标本放置时间的延长,白细胞(WBC)、红细胞(RBC)、血红蛋白(HGB)、红细胞分布宽度(RDW)、中间细胞百分率(MON%)略有变化,但无统计学意义(P>0.05);血小板(PLT)、平均血小板体积(MPV)、血小板分布宽度(PDW)、中性粒细胞百分率(GRA%)、淋巴细胞百分率(LYM%)各参数差异均有统计学意义(P<0.05),且差异主要集中在即刻到0.5小时和2小时以后。结论:幼儿末梢血标本在室温下放置时间长短对血常规检测中的一些参数有明显影响,但在0.5～2小时检测影响不大,因此建议在该时间段测定。

Objective：to know the peripheral blood of children place time routine blood test influence, to daily work reasonable arrangement work process, for clinical provide reliable experimental data. Methods： the data of 50 2 -5 years old children endings in health blood ethylenediamine tetraacetate three k （ EDTA - K3 ） anticoagulation, room temperature of（20℃ - 25℃ ） in immediate, respectively 0.5 h,2 h,4 h,8 h blood cells with automatic analyzer for testing. Results： at ambient temperature, blood specimen with endings placed the extension of time, white blood cells （ WBC ） , red blood cells （ RBC ） , the hemoglobin （HGB） , red blood cell dis- tribution width （ RDW ）, middle cells percentage （ MON % ） a little change, but this was not statistically significant （ P 〉 0.05 ） ; Platelets （ PLT ）, average platelet volume （ MPV）, platelet distribution （ PDW）, width neutrophils percentage （ GRA % ）, lymphocytes percentage （ LYM % ） parameters are statistically significant difference （ P 〈 0. 05 ）, and the difference in the immediate focus to 0.5 h and 2 h after. Conclusion ： the primary endings specimen of blood at the room temperature of placing time length of routine blood test of some parameters have obvious influence, but in 0.5 h - 2 h detection effect between little, so in this time period Suggestions were determined.