钴60γ射线灭活人工骨钉PRV病毒效果验证

Validation of porcine pesudorabies virus inactivation by gamma rays irradiation from cobalt 60

目的:验证钴60辐照灭活猪源性人工骨钉中指示病毒PRV活性的有效性。方法:首先利用CPE法测定不同辐照剂量γ射线灭活样品后指示病毒的感染活性,初步确定有效辐照剂量;然后利用三批样品对该辐照剂量灭活效果进行验证,辐照前后人工骨钉中指示病毒的滴度采用96孔板CPE法滴定。结果:CPE法测定结果显示,经20kGy及以上辐照剂量处理的样品中指示病毒PRV不能使宿主细胞PK-15出现细胞病变;三批样品经20kGy辐照剂量处理后,指示病毒PRV滴度下降值⊿lgTCID50分别>4.06,>4.75,>4.28,⊿lgTCID50值均大于4。结论:辐照剂量为20kGy的钴60γ射线辐照猪源性人工骨钉可以作为灭活PRV的有效手段。

Objective：To validate the indicating virus inactivation in artificial bone screw by Gamma Rays Irradiation from Cobalt 60. Methods： The indicating virus PRV virulence was determined with irradiation at different dosages（10kGy, 15 kGy, 20 kGy, 25 kGy） by cytopathogenic effect（CPE） method, then the effective irradiation dose to inactivate the PRV in artificial bone screw was set, three batch products were used to validate the inactivation effect next. The titer of PRV was assayed with 96-well plate by CPE method. Results： CPE results showed indicating virus PRV in artificial bone screw treated with irradiation dose at 20kGy or more, cannot make PK -15 cells have pathological changes. After irradiation, the logarithm value of PRV titer of three batch product reduced more than 4.06,4.75,4.28 respectively. The logarithm values were more than 4. Conclusion： It was an effective method to inactivate PRV in artificial bone screw with 20kGy irradiation from Cobalt 60.