摘要
利用ECMWF数值预报产品和PP法建立指标模型 ,把天气动力、定量统计、相似分析运用到中期天气预报中 。
Used ECMWF NWP products,the system is based on PPM forecast and founded index-models.It is a significant test to use weather-dynamic principles,similar analysis,quantitative statistics to the medium-range forecast. Key words:medium-range forecast;NWP productsd No.136, were selected to insert foreign epitopes. Epitopes such as VP1 of the foot mouth disease virus and the C3 epitope of poliovirus were inserted into CS3 pili. The results of whole cell ELISA, immunofluorescence and electron microscopy showed they were displayed on the surface of the recombinant cells. Mice were immunized by injecting the recombinant bacteria intraperitoneally to evaluate the immunogenicity of the hybrid proteins. The result showed that mice could produce antibody response against CS3 and the foreign epitopes. All in above indicted CS3 can surface display the foreign epitopes in multiple sites, and it may probably become an expression vector for the construction of the live gene engeneering vaccine especially multi covalent vaccine.
出处
《甘肃气象》
2000年第1期43-46,共4页
Gansu Meteorology
关键词
中期预报
数值预报产品
天气预报
PP法
CS3 pili , Antigenic epitope, Surface display, Escherichia coli