黄酮对力竭运动大鼠心肌抗氧化与细胞凋亡的影响

Effects of Flavones on Antioxidant Enzymes and Apoptosis in Heart of Exhauted Exercise Rats

以健康雄性SD大鼠为研究对象,通过建立灌胃罗汉果叶黄酮(FSGL)的干预力竭性运动大鼠,来探讨不同剂量FSGL对力竭运动大鼠心肌抗氧化酶系及心肌Bcl-2、Bax mRNA表达的影响。结果表明:不同剂量组的大鼠力竭运动后心肌SOD、CAT和GSH-PX活性与力竭运动对照组比较,均呈升高趋势,以中剂量组效果最显著;力竭运动组MDA与中剂量组比较有极显著性差异;与力竭运动组比较,各剂量组Bcl-2基因表达加强,且中剂量组显著高于力竭运动组和低剂量组。力竭运动组Bax基因表达极显著高于安静对照组和中剂量组(P<0.01),同时低剂量和高剂量组Bax的表达显著高于安静对照组(P<0.05)。FSGL各剂量组Bcl-2/Bax比值均升高,以中剂量和高剂量组最显著(P<0.01)。可见,FSGL能有效提高机体的抗氧化能力,明显地抑制因过度训练导致的心肌细胞凋亡程度。

Based on the study of health and male Sprague-Dauley rats,and by setting up the experiment model of exhausted exercise after irrigating flavones from Siraitia grosvenori Leaf（FSGL）,the effects of different dose of FSGL are explored on antioxidant enzymes and Bcl-2,Bax mRNA expression in heart of exhauted exercise rats. The result indicated that the activities of SOD,CAT,GSH-PX in myocardial tis- sue of different doseages of FSGL were higher compared with exhaustion movement control group （EMG） ,especially the middle dose flavones from Siraitia grosvenori leaf（MFSGL）;the MDA concentra- tion in EMG increased significantly compared with MFSGL. The expression of Bel-2 protein in different doseages of FSGL were strengthened compared with EMG,and MFSGL＇s expression were strengthened significantly compared with EMG and LFSGL （P〈0.05）. Meanwhile,compared with NG and MFSGL, the expression of Bax protein in EMG increased significantly （P〈0.01）. The Bcl-2/Bax ratio in the different doseages of FSGL increased,especially the MFSGL and HFSGL （P〈0. 01）. It was clear that FSGL has a good effect to enhance the antioxidation ability of organism and to inhibit the degree of cardiomyocyte apoptosis induced by exhausted exercise.