水稻OsRacD与OsRhoGDI2基因共表达载体的构建

Construction of the Co-expresstion Vector of Rice OsRhoGDI2 and OsRacD Gene

水稻OsRacD是从水稻幼穗中分离的Rho基因,OsRhoGDI2是通过酵母双杂交筛选到的与OsRacD相互作用蛋白的编码基因,已有的研究表明OsRacD与OsRhoGDI2之间可能存在相互作用,并参与水稻育性调控过程.为了鉴定二者的相互作用,本研究构建了OsRacD与OsRhoGDI2基因共表达载体,为采用免疫共沉淀验证二者在体内的相互作用,解析OsRacD与OsRhoGDI2基因参与水稻育性控制的分子机制奠定了基础.

OsRacD gene was isolated from panicle as one member of rice Rho family, and its putative partner OsRhoGDI2 gene was further obtained by yeast two-hybrid screening. Our previous study showed that OsRacD and OsRhoGDI2 may participate the rice fertility control by protein-protein interactions. In order to investigate their interactions in vivo, the co-expression vector of OsRacD and OsRhoGDI2 was constructed in this paper which facilitated the binding analysis with co-irnmunoprecipitation, and also helped to resolve their underlying molecular mechanism in rice development and fertility control.