凝固酶阴性葡萄球菌对苯扎溴铵消毒剂的抗力观察

OBSERVATION ON RESISTANCE OF COAGULASE-NEGATIVE Staphylococci TO BENZALKONIUM BROMIDE DISINFECTANT

目的研究医院环境分离凝固酶阴性葡萄球菌分布及其对苯扎溴铵消毒液抗力的变化情况。方法采用细菌检测和肉汤稀释法,对医院环境标本进行了细菌分离鉴定并测定分离的凝固酶阴性葡萄球菌对苯扎溴铵的最小抑菌浓度(MIC)。结果从841份医院环境表面和医护人员手标本中共分离出63株凝固酶阴性葡萄球菌,分离率为7.49%;从中检出12株携带qacA基因的凝固酶阴性葡萄球菌。苯扎溴铵对携带qacA基因的12株凝固酶阴性葡萄球菌MIC值范围为0.7 mg/L～2.8 mg/L,其中11株的MIC值均高于金黄色葡萄球菌标准株。结论携带qacA基因的凝固酶阴性葡萄球菌对苯扎溴铵的抗力普遍高于金黄色葡萄球菌消毒试验标准菌株。

Objective To study the distribution of hospital isolated coagulase-negative staphylococci and resistance change to benzalkonium bromide disinfectant.Methods Bacterial detection and broth dilution method were used to isolate and identify the hospital isolated coagulase-negative staphylococci and determine the minimum inhibitory concentration（MIC） of benzalkonium bromide.Results From the 841 samples of the hospital environment surfaces and health care personnel hand specimens,63 strain of coagulase-negative staphylococci were isolated,the separation rate of 7.49%.Of them 12 strain of coagulase-negative staphylococci carryied the qacA gene.The MIC values was range from 0.7 mg/L to 2.8 mg/L of benzalkonium bromide to 12 coagulase-negative staphylococci carried qacA gene,11 of MIC values were higher than Staphylococcus aureus standard strains.Conclusion The resistance of coagulase-negative staphylococci carried qacA gene to benzalkonium bromide generally higher than the standard strains of Staphylococcus aureus.