石墨炉原子吸收光谱法测定海产品砷含量

GFAAS Determination of Arsenic in Seafood

石墨炉原子吸收光谱法是一种操作简便、灵敏度高、检出限低的元素测定方法,但测砷时有较为严重的基体干扰。本研究采用不同基体改进剂以消除基体干扰,优化了原子化温度,对比了不同的消解方法,建立了海洋产品中砷的石墨炉原子吸收的测定方法。最佳原子化温度为2750℃,以氯化钯与硝酸镍混合液为基体改进剂,可以得到较好结果。高氯酸浓度升高,虾粉的测量结果下降,盐酸浓度小于等于1%,对虾粉的测定结果影响不显著,达到3%后,虾粉的测定结果显著下降。砷与钠、钾、钙、镁的比例为1∶400,结果显示离子没有干扰,砷与铁的比例为1∶200,结果显示没有干扰。

Graphite furnace atomic absorption spectrometry（GFAAS） is an easy,high-sensitive and low-detection-limit method for detection of chemical elements.However,serious interference of matrix sometimes occurs when this assay is applied on arsenic detection.In this study we established a method for detection of the arsenic content in seafood via GFAAS by using a variety of matrix modifiers to eliminate interference of matrix,optimizing the atomization temperature and evaluating different digestion methods.It was indicated that nice result could be obtained at the optimal atomization temperature as 2750 ℃ and with the mixture of palladium chloride and nickel nitrate as matrix modifier.This method was then evaluated by arsenic detection in shrimp powder.The result showed that the value of arsenic content went down as the concentration of perchloric acid went up,and that variation of the value was insignificant when the concentration of hydrochloric acid was lower than 1 %,whereas significantly decreased when the concentration ranged from 1 % to 3 %.Furthermore,no interference of matrix was observed when the concentration ratio of arsenic to sodium,potassium,calcium or magnesium was 1：400,and when the ratio of arsenic to ferrum（iron） was 1：200.