艾炷灸对动脉粥样硬化兔主动脉内皮细胞超微结构的影响

Effects of Moxa Cone Moxibustion on Ultrastructure of Aorta Endotheliocyte in Rabbits with Atherosclerosis

[目的]通过观察兔主动脉内皮细胞超微结构的变化,探讨艾炷灸对动脉粥样硬化(AS)兔血管内皮细胞的影响。[方法]将60只新西兰大耳白兔随机分为4组,每组15只,即空白组(空白对照组),模型组(AS模型组),艾炷灸组(AS模型+艾炷灸),西药组(AS模型+阿托伐他汀)。通过高脂饲养加免疫损伤的方法建立AS模型,并且艾炷灸组和西药组施以相应的干预措施。喂饲16周后检测血脂指标(TG、LDL-CH),透射电镜下观察主动脉内皮细胞超微结构的病理变化。[结果]与模型组比较,艾炷直接灸组和西药组TG、LDL-CH含量明显降低(P<0.05或P<0.01),且内皮细胞形态较正常,内弹力膜连续,中膜平滑肌细胞基本正常。[结论]艾炷直接灸有较好的调节血脂代谢、抗AS及改善血管内皮细胞损伤的作用。

[ Objective ] To discuss the effects of moxa cone moxibustion on vascular endothelial cells in rabbits with atherosclerosis through the observation of ultrastructure changes of aorta endotheliocyte. [ Methods ] 60 New Zealand white rabbits were randomly divided into 4 groups ： blank group （ control group） , model group （ AS model group） , moxibustion group （AS model plus moxa cone moxibustion） and western medicine group （AS model plus atorvastatin） ,15 rabbits per group. AS model of rabbit was established by using hypercholesterol diet combined with immunologic injury, and moxa cone moxibustion group and Western medicine group were treated with corresponding intervention measures. Blood lipid indexes （ TG, LDL - CH） were detected and ul- trastrncture pathological changes of aorta endotheliocyte were observed under transmission electron microscope after feeding for 16 weeks. [ Results ] Compared with the model group, contents of TG and LDL -CH in western medicine group and direct moxibustion group was obviously decreased （ P 〈 0.05/P 〈 0.01 ） , and the morphouses of endothelial ceils and media smooth muscle cells were almost normol, the internal elastic membranes were continuous. [ Conclusion ] Moxa cone moxibustion has better effects of regulating blood lipid metabolism and improving injury of endothelial cells.