慢性无症状HBV携带者阴虚体质与人类白细胞抗原-DRB1、DQA1基因多态性的联系

Study on the Correlation between Chronic Asymptomatic HBV Carriers of Yin Asthenia Constitution and Genotypes of HLA-DRB1 and HLA DQA1 Alleles

目的探讨慢性无症状HBV携带者(chronic asymptomatic HBV carrier,ASC)阴虚体质与人类白细胞抗原(human leukocyte antigen,HLA)-DRB1、HLA-DQA1基因多态性的内在联系。方法将105例ASC患者进行中医体质分型及分组:阴虚质组47例和非阴虚质组58例。采用聚合酶链反应-序列特异性引物(PCR-SSP)技术测定HLA-DRB1、HLA-DQA1等位基因型。结果阴虚质组HLA-DRB1*09、HLA-DQA1*0301的基因频率明显低于非阴虚质组(分别为12.1%vs27.8%,19.1%vs39.7%),差异有统计学意义(P<0.01);HLA-DRB1*11、HLA-DQA1*0501的基因频率明显高于非阴虚质组(分别为12.1%vs4.3%,28.7%vs9.5%),差异有统计学意义(P<0.05,P<0.01)。结论 HLA-DRB1*09和HLA-DQA1*0301可能是ASC非阴虚体质患者的分子基础;HLA-DRB1*11和HLA-DQA1*0501可能是ASC阴虚体质患者的分子基础。

Objective To study on the correlation between chronic asymptomatic HBV carriers （ASC） of yin asthenia constitution and genotypes of HLA-DRB1 and HLA DQA1 alleles. Methods Totally 105 ASC were assigned to two groups according to their constitutions, i. e., the yin asthenia group （47 cases） and the non-yin asthenia group （58 cases）. The genotypes of HLA-DRB1 and HLA DQA1 alleles were determined using PCR- SSP, Results The gene frequency of HLA-DRB1 ~ 09 allele and HLA-DQA1 ＊ 0301 allele （being 12. 1% and 19.1% ） were obviously lower in the yin asthenia group than in the non-yin asthenia group （ being 27. 8% and 39.7%, P〈0.05）. The gene frequency of HLA-DRB1 ＊ 11 allele and HLA-DQA1 ＊ 0501 allele were obviously higher in the yin asthenia group （being 12. 1% and 28.7%） than in the non-yin asthenia group （4.3% and 9.5%）, showing statistical difference （ P 〈 0.05, P 〈 0.01 ）. Conclusions HLA-DRB1 ＊ 09 allele and HLA-DQA1 ＊ 0301 allele might be the molecular bases for non-yin asthenia patients with ASC. HLA-DRB1 ＊ 11 allele and HLA-DQA1 ＊ 0501 allele might be the molecular bases for yin asthenia patients with ASC.