降糖消脂片对转基因小鼠降糖降脂作用的实验研究

Experimental Study of Jiangtang Xiaozhi Tablet on Decreasing the Levels of Blood Glucose and Serum Lipids in Transgenic Mice with Diabetes Mellitus

目的建立糖尿病肥胖小鼠模型,观察降糖消脂片对其血糖、血脂等指标的影响。方法采用KK-Ay转基因小鼠,高脂饲料喂养,造成糖尿病肥胖小鼠模型。对照组为同龄C57小鼠,成模的KK-Ay小鼠分为:模型组、吡格咧酮组(8mg/kg)、降糖消脂片大剂量组(简称降糖消脂大组,10g/kg)、降糖消脂片中剂量组(简称降糖消脂中组,5g/kg)、降糖消脂片小剂量组(简称降糖消脂小组,2.5g/kg),每组11只。灌胃给药8周。每周称体重及摄食量,隔周测血糖(FBG)。药后8周测FBG、总胆固醇(TC)、甘油三酯(TG)、胰岛素(INS)、瘦素(Lep);取肝脏、脂肪称重,计算其脏器指数;并对肝脏的脂肪变性进行分级、计数胰岛数目及脂肪细胞数。结果与模型组比较,降糖消脂大、中、小组体重明显减轻、摄食量明显下降(P<0.05,P<0.01);降糖消脂大组从第6周开始,FBG明显下降(P<0.01);连续灌胃给药8周后,降糖消脂大、中、小组的TG、INS明显下降(P<0.05,P<0.01);降糖消脂大组Lep有所下降(P<0.05);降糖消脂大、中、小组肝脏和脂肪指数明显降低、胰岛数目明显增多(P<0.05,P<0.01);降糖消脂大、中组的脂肪细胞数目明显减少(P<0.05,P<0.01);降糖消脂大组肝脏的脂肪变性明显改善(P<0.05)。结论降糖消脂片对KK-Ay糖尿病肥胖小鼠有明显的降糖、降脂作用。

Objective To observe the effects of Jiangtang Xiaozhi Tablet （JTXZT） on decreasing the lev- els of blood glucose and serum lipids in KK-Ay transgenic model mice with diabetes mellitus （DM）. Methods KK- Ay transgenic mice were fed with high fat diet to induce DM obesity model. The C^57 mice of the same age were re- cruited as the control group. Then the modeled KK-Ay mice were divided into 5 groups, i. e., the model group, the Pioglitazone Hydrochloride Tablet treatment group （8 mg/kg, as the positive control group）, and the high dose JTXZT group （10 g/kg）, the middle dose JTXZT group （5 g/kg）, and the low dose JTXZT group （2.5 g/ kg）, 11 in each group. Eight weeks was taken as one therapeutic course. All medicines were given by gastroga- vage. The food intake and body weight were measured every week. The blood glucose level was determined once every 2 weeks. The levels of fasting blood glucose （FBG）, serum lipids （TC and TG）, insulin （INS）, and leptin （Lep） were assayed 8 weeks after medication. The weight of liver and fat were weighed to calculate their indices. Then the adipose denaturalization of the liver was classified. The numbers of the pancreatic islet and adi- pocytes were respectively counted. Results Compared with the model group, the food intake and the body weight obviously decreased in the 3 JTXZT groups （P〈0. 05, P〈0. 01 ）. From the 6th week, the FBG level ob- viously decreased in the high dose JTXZT group （ P 〈0.01 ）. After eight successive weeks of intragastric admin- istration, the levels of TG and INS obviously decreased in the 3 JTXZT groups （P〈0. 05, P〈0. 01 ）. The Lep lev- el somewhat decreased in the high dose JTXZT group （ P 〈0. 05）. The indices of the liver and fat obviously de-creased and the number of the pancreatic islet obviously increased in the 3 JTXZT groups （ P 〈 0. 05, P 〈 0. 01 ）. The number of adipocytes obviously decreased in the high and middle dose JTXZT groups （ P 〈0. 05, P 〈0. 01 ）. The liver fatty degeneration was obviously improved in the high dose JTXZT group （P〈0. 05）. Conclusion JTXZT had obvious effects on decreasing the levels of blood glucose and serum lipids in KK-Ay transgenic model mice with DM obesity.