miRNAlet7、Ras在非小细胞肺癌中的表达及其临床意义

Studies on the expressions and clinical significance of miRNAlet 7 and Ras in non-small cell lung cancer

目的探讨miRNAlet7（1et7）、Ras在非小细胞肺癌中的表达及临床病理学意义。方法利用原位杂交方法检测let7、免疫组化方法检测Ras在68例非小细胞肺癌（肺癌组）及20例癌旁正常肺组织（对照组）中的表达。结果let7在肺癌组中的阳性表达率（39．7％）低于对照组（75．0％）（P〈0．05）、Ras在肺癌组中的阳性表达率（66．2％）高于对照组（25．0％）（P〈0．01）；let7阳性率与患者年龄、性别及肿瘤组织类型、分化程度、分期无关（P〉0．05），Ras阳性率与吸烟史、患者性别及肿瘤组织类型有关（P〈0．01），与肿瘤分化程度、淋巴结转移及临床分期无关（P〉0．05）；let7与Ras在非小细胞肺癌组织中表达呈明显负相关（r=-0．627，P〈0．01）；let7阳性表达组2年生存率明显优于阴性表达组（X2=4．84，P〈0．05）；Ras阳性表达与阴性表达组2年生存率差异无统计学意义（P〉0．05）。结论let7的低表达、Ras的高表达参与了肺癌的发生发展，let7阳性表达与预后密切相关；Ras可协同参与肺癌的发生发展。

Objective To study the expressions of miRNAlet7 and Ras in non-small cell lung cancer （NSCLC）, and their correlations with clinicopathological features and survival time. Methods In-situ hybridization was used to detect the expression of let7, and SP immunohistochemistry to measure HMGA2 in 68 NSCLC cases （group A） and 20 cases with normal lungs （group B）. Results The positive rate of let7 in group A was lower than that in group B （39. 7% vs 63.2% ） （ P 〈0. 05） . The positive rate of Ras in group A was higher than that in group B （66.2% vs 25.0% ） （ P 〈0.01 ） . The positive rate of let7 was not related to the age, gender, histological type, cell differentiation, and clinical stages of cancer patients （ P 〉0. 05）. The positive rate of Ras was related to smoking, sex, and histological type of cancer（ P 〈 0. 01 ）, and was not related to cell differentiation, lymphatic metastasis, and clinical stages of cancer （ P 〉 0. 05）. There was an obvious negative correlation between let＇/and Ras（ r = -0.627, P 〈0. 01）. The 2- year survival rate of let7-positive group was higher than that of the let 7-negative group （ X2 = 4. 84, P 〈 0. 05 ）. No statistically significant difference was found between Ras-positive and -negative groups （ P 〉 0. 05 ）. Conclusions The lower level of let7 expression, and high level of Ras expression have much to do with the carcinogenesis of NSCLC. The level of let7-positive expression is closely related to prognosis ; while Ras may act in cooperativity in the occurrence and development of NSCLC.