枳棋子水提取液对乙醇体外诱导脂肪变L-02细胞UCP2基因表达的影响

Effect of Hovenia Dulcis Thunb liquid on the expression of UCP2 gene in ethanol-induced superfatted L-02 cells in vitro

目的通过建立乙醇体外诱导的L-02肝细胞脂肪肝模型，探讨不同剂量的乙醇对肝脏脂肪变L-02细胞解偶联蛋白（Uncoupling protin2，UCP2）表达水平的关系，观察枳棋子水提取液对其表达的影响。方法建立肝细胞脂肪变模型，选最适合浓度枳棋子提取液进行干预，采用半定量RT．PCR法测各组UCP2mRNA表达量。结果各浓度脂肪变性模型中UCP2mRNA表达均有下降，经枳棋子水提取液干预后UCP2mRNA表达均有明显升高（0．6％乙醇组治疗后值由0．4859升至0．7442）。结论乙醇可降低L-02细胞UCP2mRNA的表达，UCP2mRNA的低表达可能与L-02细胞AFLD发生有关。枳棋子可使L-02细胞UCP2mRNA的表达上调，并推测UCP2mRNA的表达上调是枳棋子减轻酒精诱导肝细胞脂肪变性的机制之一。

Objective To establish the model of alcohol-induced hepatocyte steatosis in L-02 liver cells in vitro, investigate the relationship between cell damage, UCP2 gene expression and ethanol concentrations in L-02 cells of steatosis, and explore the protective effect of Hovenia Dulcis Thunb extracted liquid. Method Hovenia Dulcis Thunb extracted liquid at the most compatible dosage was applied to the L- 02 cells of steatosis. Then UCP2 mRNA was detected by the semi-quantitative RT-PCR method. Results In steatosis model groups, UCP2 mRNA expression was downregulated （ P 〈 0. 05）. In the steatosis groups treated with Hovenia I）ulcis Thunb extracted liquid, UCP2 mRNA level was significantly increased compared to steatosis group （ its expression level increased from 0. 4859 to 0. 7442 after treatment of 0. 6% ethanol, P 〈 0. 05）. Conclusions Ethanol can down-regulate the UCP2 mRNA expression of L-02 cells in a certain period. The low expression of UCP2 mRNA is probably relevant to the occurrence of alcoholic fatty liver dis- ease. Hovenia Dulcis Thunb can prevent from the cell toxicity of ethanol and decrease the degree of steatosis. Hovenia Dulcis Thunb extracted liquid can increase the expression level of UCP2 mRNA in the L-02 ceils.